DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, SE | - |
dc.contributor.author | Kang, H | - |
dc.contributor.author | Shim, GY | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Choi, HG | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Hahn, SK | - |
dc.contributor.author | Oh, YK | - |
dc.date.accessioned | 2017-07-18T16:56:32Z | - |
dc.date.available | 2017-07-18T16:56:32Z | - |
dc.date.created | 2009-08-24 | - |
dc.date.issued | 2009-02 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/31068 | - |
dc.description.abstract | In this study, we tested the use of cationic polymer derivatives of biocompatible hyaluronic acid (HA) as a delivery system of siRNA and antisense oligonucleotides. HA was modified with cationic polymer polyethylenimine (PEI). When compared with PEI alone, cationic PEI derivatives of HA (HA-PEI) provided increased cellular delivery of Small interfering RNA (siRNA) in B16F1, A549, HeLa, and Hep3B tumor cells. Indeed, more than 95% of the cells were positive for siRNA following its delivery with HA-PEI. A survivin-specific siRNA that was delivered using HA-PEI potently reduced the mRNA expression levels of the target gene in all of the cell lines. By contrast, survivin-specific siRNA delivered by PEI alone did not induce a significant reduction in mRNA levels. In green fluorescent protein (GFP)-expressing 293 T cells, a loss of GFP expression was evident in the cells that had been treated with GFP-specific siRNA and HA-PEI complex. The inhibition of target gene expression by antisense oligonucleotide 63139 was also enhanced after delivery with HA-PEI. Moreover, HA-PEI displayed lower cytotoxicity than PEI alone. These results suggest that HA-PEI could be further developed as biocompatible delivery systems of siRNA and antisense oligonucleotides for enhanced cellular uptake and inhibition of target gene expression. | - |
dc.language | English | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.title | Cationic derivatives of biocompatible hyaluronic aicd for delivery of siRNA and antisence oligonucleotides | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/10611860802472461 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.17, no.2, pp.123 - 132 | - |
dc.identifier.wosid | 000266593200004 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.endPage | 132 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 123 | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 17 | - |
dc.contributor.affiliatedAuthor | Hahn, SK | - |
dc.identifier.scopusid | 2-s2.0-58149473080 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 35 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CELLULAR UPTAKE | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Hyaluronic acid | - |
dc.subject.keywordAuthor | cationic polymer | - |
dc.subject.keywordAuthor | siRNA delivery | - |
dc.subject.keywordAuthor | antisense oligonucleotides | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
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