DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang H.S | - |
dc.contributor.author | Shin G.W | - |
dc.contributor.author | Chung B | - |
dc.contributor.author | Na J | - |
dc.contributor.author | Jung G.Y. | - |
dc.date.accessioned | 2017-07-19T09:16:24Z | - |
dc.date.available | 2017-07-19T09:16:24Z | - |
dc.date.created | 2012-09-28 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 1940-6029 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/34665 | - |
dc.description.abstract | Among the molecular diagnostic methods for bacteria-induced diseases, capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR has enormous potential because it can separate sequence variants using a simple procedure. However, conventional CE-SSCP systems have limited resolution and cannot separate most 16S rRNA gene-specific markers into separate peaks. A high-resolution CE-SSCP system that uses a poly(ethyleneoxide)-poly(propyleneoxide)- poly(ethyleneoxide) triblock copolymer matrix was recently developed and shown to effectively separate highly similar PCR products. In this report, a protocol for the detection of 12 pathogenic bacteria is provided. Pathogen markers were amplified by PCR using universal primers and separated by CE-SSCP; each marker peak was well separated at baseline and showed a characteristic mobility, allowing the easy identification of the pathogens. | - |
dc.language | English | - |
dc.publisher | Humana press | - |
dc.relation.isPartOf | Methods in Molecular Biology | - |
dc.title | Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single-Strand Conformation Polymorphism | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/978-1-62703-029-8_15 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Methods in Molecular Biology, v.919, pp.155 - 163 | - |
dc.citation.endPage | 163 | - |
dc.citation.startPage | 155 | - |
dc.citation.title | Methods in Molecular Biology | - |
dc.citation.volume | 919 | - |
dc.contributor.affiliatedAuthor | Jung G.Y. | - |
dc.identifier.scopusid | 2-s2.0-84934441984 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | ARTICLE | - |
dc.description.journalRegisteredClass | scopus | - |
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