DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SB | - |
dc.date.accessioned | 2017-07-19T12:14:53Z | - |
dc.date.available | 2017-07-19T12:14:53Z | - |
dc.date.created | 2016-01-15 | - |
dc.date.issued | 2015-08 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/35506 | - |
dc.description.abstract | Recently, Yun et al. [Envron. Microbiol. 17: 1677-1688 (2015)] proposed a metabolic pathway of 3,6- anhydro-L-galactose (L-AnG) in Vibrio sp. EJY3. The sequence of Yun's pathway is 3,6-anhydro-L-galactose -> 3,6-anhydro-galactonate -> 2-keto-3-deoxy-galactonate -> DeLey-Doudoroff pathway. This pathway differs significantly from one that has been established by Lee et al. [Biotechnol. Bioproc. Eng. 19: 866-878 (2014)], and which has been detected in agar-degrading bacteria such as Postechiella marina M091, Pseudoalteromonas atlantica T6c, and Streptomyces coelicolor A3(2). The sequence of Lee's pathway is 3,6-anhydro-L-galactose -> 3,6-anhydro-Lgalactonate -> 2-keto-3-deoxy-L-galactonate -> 2,5-diketo- 3-deoxy-L-galactonate -> 2-keto-3-deoxy-D-gluconate -> 2-keto-3-deoxy-6-phospho-D-gluconate -> pyruvate + Dglyceraldehyde- 3-phosphate. Because Yun's L-AnG pathway is connected to the DeLey-Doudoroff pathway and 2-keto- 3-deoxy-D-galactonate (D-KDGal) is an intermediate in the DeLey-Doudoroff pathway, the 2-keto-3-deoxy-galactonate in the Yun's pathway must be D-KDGal. On the contrary, Lee et al. showed that the product of the reaction catalyzed by the second-step enzyme is 2-keto-3-deoxy-L-galactonate (L-KDGal). The E. coli genome contains the genes that enable conversion of D-KDGal to glycolysis intermediates via the DeLey-Doudoroff pathway. However, if the reaction product of the second-step enzyme is L-KDGal, additional genes are required for the conversion of L-KDGal to glycolysis intermediates. Here I argue that the validity of the following claims by Yun et al. is very questionable: (1) the formation of D-KDGal during the metabolism of L-AnG in Vibrio sp. EJY3 and (2) the production of ethanol from L-AnG using the DeLey-Doudoroff pathway in E. coli. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.title | Unusual metabolism of 3,6-anhydro-L-galactose in Vibrio sp EJY3 and in E-coli containing two Vibrio sp EJY3 genes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/S12257-015-0440-2 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.20, no.4, pp.714 - 717 | - |
dc.identifier.wosid | 000361878400011 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.endPage | 717 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 714 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Lee, SB | - |
dc.identifier.scopusid | 2-s2.0-84942520201 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.description.scptc | 2 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RED MACROALGAE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | SUPERFAMILY | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | MARINE | - |
dc.subject.keywordAuthor | metabolism | - |
dc.subject.keywordAuthor | 3,6-anhydro-L-galactose | - |
dc.subject.keywordAuthor | Postechiella marina M091 | - |
dc.subject.keywordAuthor | Pseudoalteromonas atlantica T6c | - |
dc.subject.keywordAuthor | Streptomyces coelicolor A3(2) | - |
dc.subject.keywordAuthor | Vibrio sp EJY3 | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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