DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo, HY | - |
dc.contributor.author | Song, YH | - |
dc.contributor.author | Seo, JH | - |
dc.contributor.author | Cha, HJ | - |
dc.contributor.author | HWANG, DONG SOO | - |
dc.date.accessioned | 2017-07-19T12:15:15Z | - |
dc.date.available | 2017-07-19T12:15:15Z | - |
dc.date.created | 2016-01-15 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/35521 | - |
dc.description.abstract | Endurable coating on biomedical substrates is one of the most important issues in biomedical engineering field. Recently, a robust yet reversible coating has found in the cuticle of mussel byssus and the mimicking of the mussel cuticle coating has been considered as a strategy to build up endurable coating on biomedical materials. To date, the only known protein in the cuticle is mussel foot protein-1 (fp-1). To form endurable and bioactive coating on the biomedical surface, the fusion protein of fp-1 and GRGDSP peptide (fp-1-RGD) was genetically designed and produced in E. coli. The fusion protein of fp-1-RGD was successfully expressed as a form of inclusion body and was simply purified by diluted acetic acid extraction with high purity (similar to 95%). Fp-1-RGD was coated on the tissue culture polystyrene (TCPS) and showed better preosteoblast cell proliferation than that of TCPS. Therefore, the marriage of fp-1 and bioactive peptide can be a good strategy to form bioactive and endurable coating in biomedical field. | - |
dc.language | English | - |
dc.publisher | The Korean Society for Biotechnology and Bioengineering | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.title | Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/S12257-014-0621-4 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.20, no.2, pp.211 - 217 | - |
dc.identifier.wosid | 000354711700002 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.endPage | 217 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 211 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.contributor.affiliatedAuthor | HWANG, DONG SOO | - |
dc.identifier.scopusid | 2-s2.0-84929464880 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.description.scptc | 2 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MYTILUS-EDULIS-L | - |
dc.subject.keywordPlus | ATTACHMENT PLAQUE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MATRIX PROTEINS | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER | - |
dc.subject.keywordPlus | BYSSAL THREADS | - |
dc.subject.keywordPlus | COLLAGEN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INTEGRINS | - |
dc.subject.keywordPlus | RGD | - |
dc.subject.keywordAuthor | biomedical coating material | - |
dc.subject.keywordAuthor | mussel coating protein | - |
dc.subject.keywordAuthor | RGD peptide | - |
dc.subject.keywordAuthor | cell recognition motif | - |
dc.subject.keywordAuthor | fusion protein | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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