A nano-scale probing system with a gold nano-dot array for measurement of a single biomolecular interaction force
SCIE
SCOPUS
- Title
- A nano-scale probing system with a gold nano-dot array for measurement of a single biomolecular interaction force
- Authors
- Kim, SJ; Seo, JH; Lee, JW; Cho, DW; Cha, HJ; Moon, W
- Date Issued
- 2015-01
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- A new nano-scale probing system was proposed and developed to measure and analyze the interaction force between biomolecules at the single molecular level. A probing instrument adopting the working principle of atomic force microscopy (AFM) was designed to measure a single biomolecular interaction force with a patterned gold nano-dot array and functionalized AFM probe. The constructed probing system was evaluated through measurement of the unbinding force between a protein (Vibrio cholerae toxin B subunit) and a carbohydrate (GM1 pentasaccharide) as a model single biomolecular interaction. Thus, the developed probing system might be a potential platform for the effective measurement of single biomolecular level interactions.
- Keywords
- SURFACE-PLASMON RESONANCE; SPECTROSCOPY; MICROSCOPE; MOLECULES; GLYCOSYLATION; SPECIFICITY; AFFINITY; DNA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/35717
- DOI
- 10.1039/C5RA23186H
- ISSN
- 2046-2069
- Article Type
- Article
- Citation
- RSC ADVANCES, vol. 5, no. 128, page. 105727 - 105730, 2015-01
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- There are no files associated with this item.
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