DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, CS | - |
dc.contributor.author | Seo, JH | - |
dc.contributor.author | Cha, HJ | - |
dc.date.accessioned | 2017-07-19T12:38:43Z | - |
dc.date.available | 2017-07-19T12:38:43Z | - |
dc.date.created | 2016-02-26 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/36181 | - |
dc.description.abstract | Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.title | Functional characterization of Vibrio cholerae O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/S12257-015-0677-9 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.20, no.6, pp.980 - 987 | - |
dc.identifier.wosid | 000368366700002 | - |
dc.date.tcdate | 2018-03-23 | - |
dc.citation.endPage | 987 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 980 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-84954507743 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.scptc | 0 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENTEROBACTERIAL COMMON ANTIGEN | - |
dc.subject.keywordPlus | SIGNAL RECOGNITION PARTICLE | - |
dc.subject.keywordPlus | C-TERMINAL DOMAIN | - |
dc.subject.keywordPlus | SALMONELLA-ENTERICA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MEMBRANE-PROTEINS | - |
dc.subject.keywordPlus | MANNOSYLTRANSFERASE PIMA | - |
dc.subject.keywordPlus | TRANSFERASE | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | POLYSACCHARIDE | - |
dc.subject.keywordAuthor | WbeW enzyme | - |
dc.subject.keywordAuthor | Vibrio cholerae O1 | - |
dc.subject.keywordAuthor | undecaprenyl phosphate galactose-1-phosphate transferase | - |
dc.subject.keywordAuthor | membrane protein | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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