DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, JH | - |
dc.contributor.author | Hyung Woo Kim | - |
dc.contributor.author | Kyoung Je Cha | - |
dc.contributor.author | Jiyou Han | - |
dc.contributor.author | Yu Jin Jang | - |
dc.contributor.author | Kim, DS | - |
dc.contributor.author | Kim, JH | - |
dc.date.accessioned | 2017-07-19T12:47:42Z | - |
dc.date.available | 2017-07-19T12:47:42Z | - |
dc.date.created | 2016-04-17 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/36453 | - |
dc.description.abstract | Although previous studies suggest that nano topographical features influence properties and behaviors of stem cells, only a few studies have attempted to derive clinically useful somatic cells from human pluripotent stem cells using nanopatterned surfaces. In the present study, we report that polystyrene nanopore-patterned surfaces significantly promote the pancreatic differentiation of human embryonic and induced pluripotent stem cells. We compared different diameters of nanopores and showed that 200 nm nanopore-patterned surfaces highly upregulated the expression of PDX1, a critical transcription factor for pancreatic development, leading to an approximately 3-fold increase in the percentage of differentiating PDX1(+) pancreatic progenitors compared with control flat surfaces. Furthermore, in the presence of biochemical factors, 200 nm nanopore-patterned surfaces profoundly enhanced the derivation of pancreatic endocrine cells producing insulin, glucagon, or somatostatin. We also demonstrate that nanopore-patterned surface-induced upregulation of PDX1 is associated with downregulation of TAZ, suggesting the potential role of TAZ in nanopore-patterned surface-mediated mechano-transduction. Our study suggests that appropriate cytokine treatments combined with nanotopographical stimulation could be a powerful tool for deriving a high purity of desired cells from human pluripotent stem cells. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | ACS NANO | - |
dc.title | Nanotopography Promotes Pancreatic Differentiation of Human Embryonic Stem (ES) Cells and Induced Pluripotent Stem (iPS) Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/ACSNANO.5B06985 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ACS NANO, v.10, no.3, pp.3342 - 3355 | - |
dc.identifier.wosid | 000372855400035 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 3355 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 3342 | - |
dc.citation.title | ACS NANO | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Kim, DS | - |
dc.identifier.scopusid | 2-s2.0-84961875440 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 22 | - |
dc.description.scptc | 16 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HEPATOCYTE-LIKE CELLS | - |
dc.subject.keywordPlus | BETA-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DEFINITIVE ENDODERM | - |
dc.subject.keywordPlus | ENDOCRINE PANCREAS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordAuthor | polystyrene nanopore surfaces | - |
dc.subject.keywordAuthor | nanoinjection molding | - |
dc.subject.keywordAuthor | human embryonic stem cells | - |
dc.subject.keywordAuthor | induced pluripotent stem cells | - |
dc.subject.keywordAuthor | pancreatic differentiation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
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