DC Field | Value | Language |
---|---|---|
dc.contributor.author | Heo, KS | - |
dc.contributor.author | Ryoo, SW | - |
dc.contributor.author | Kim, L | - |
dc.contributor.author | Nam, M | - |
dc.contributor.author | Baek, ST | - |
dc.contributor.author | Lee, H | - |
dc.contributor.author | Lee, AR | - |
dc.contributor.author | Park, SK | - |
dc.contributor.author | Park, Y | - |
dc.contributor.author | Myung, CS | - |
dc.contributor.author | Kim, DU | - |
dc.contributor.author | Hoe, KL | - |
dc.date.accessioned | 2017-07-19T13:28:56Z | - |
dc.date.available | 2017-07-19T13:28:56Z | - |
dc.date.created | 2017-02-07 | - |
dc.date.issued | 2008-11-30 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/37064 | - |
dc.description.abstract | Low-density lipoprotein (LDL) induces cell proliferation in human aortic smooth muscle cells (hAoSMCs), which may be involved in atherogenesis and intimal hyperplasia. Recent studies have demonstrated that CI- channels are related to vessel cell proliferation induced by a variety of stimuli. In this study, we investigated a potential role of CI(-)channels in the signaling pathway of LDL effects on hAoSMC proliferation with a focus on the activation of Erk1/2-PI3K/Akt and the subsequent upregulation of Egr-1. CI- channel blockers, DIDS, but neither NPPB nor Furosemide, completely abolished the LDL-induced DNA synthesis and cell proliferation. Moreover, DIDS, but not NPPB, significantly decreased LDL-stimulated CI- concentration, as judged by flow cytometry analysis using MQAE as a CI--detection dye. DIDS pretreatment completely abolished the activation of Erk1/2 and PI3K/Akt in a dose-dependent manner that is the hallmark of LDL activation, as judged by Western blot and proliferation assays. Moreover, pretreatment with DIDS (CI- channel blockers) but not LY294002 (PI3K inhibitors) completely abolished the LDL-induced upregulation of Egr-1 to the same extent as PD98059 (MEK inhibitors to inhibit Erk), as judged by Western blot and luciferase reporter assays. This is the first report, to our knowledge, that DIDS-sensitive Cl-channels play a key role in the LDL-induced cell proliferation of hAoSMCs via the activation of Erk1/2 and PI3K/Akt and the upregulation of Egr-1. | - |
dc.language | English | - |
dc.publisher | Korean Society for Molecular and Cellular Biology | - |
dc.relation.isPartOf | Molecules and cells | - |
dc.title | Cl- -channel is essential for LDL-induced cell proliferation via the activation of Erk1/2 and PI3k/Akt and the upregulation of Egr-1 in human aortic smooth muscle cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Molecules and cells, v.26, no.5, pp.468 - 473 | - |
dc.identifier.wosid | 000261741600008 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 473 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 468 | - |
dc.citation.title | Molecules and cells | - |
dc.citation.volume | 26 | - |
dc.contributor.affiliatedAuthor | Baek, ST | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 7 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LOW-DENSITY-LIPOPROTEIN | - |
dc.subject.keywordPlus | GROWTH-RESPONSE GENE-1 | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject.keywordPlus | CHLORIDE CHANNELS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | CL-CURRENTS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordAuthor | CI(-)channel | - |
dc.subject.keywordAuthor | DIDS | - |
dc.subject.keywordAuthor | Egr-1 | - |
dc.subject.keywordAuthor | low-density lipoprotein | - |
dc.subject.keywordAuthor | PI3K | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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