DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, HG | - |
dc.contributor.author | Noh, MH | - |
dc.contributor.author | Jeong, JH | - |
dc.contributor.author | Park, S | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2017-07-19T13:56:26Z | - |
dc.date.available | 2017-07-19T13:56:26Z | - |
dc.date.created | 2017-02-28 | - |
dc.date.issued | 2016-11 | - |
dc.identifier.issn | 2161-5063 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/37899 | - |
dc.description.abstract | 3-Hydroxypropionic acid (3-HP) can be biologically produced from glycerol by two consecutive enzymatic reactions, dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. The pathway has been proved efficient, but imbalance between the rates of the two enzymatic reactions often results in the accumulation of the toxic 3-HPA, which severely reduces cell viability and 3-HP production. In this study, we used UTR engineering to maximally increase the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled to avoid the accumulation of 3-HPA by varying expression of dhaB1, a gene encoding a main subunit of GDHt. The optimally balanced E. coil HGL_DBK4 showed a substantially enhanced 3-HP titer and productivity compared with the parental strain. The yield on glycerol, 0.97 g 3HP/g glycerol, in a fed-batch experiment, was the highest ever reported. | - |
dc.language | English | - |
dc.publisher | ACS Publications | - |
dc.relation.isPartOf | ACS Synthetic Biology | - |
dc.title | Optimum Rebalancing of the 3-Hydroxypropionic Acid Production Pathway from Glycerol in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/ACSSYNBIO.5B00303 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ACS Synthetic Biology, v.5, no.11, pp.1247 - 1255 | - |
dc.identifier.wosid | 000388428100008 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1255 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1247 | - |
dc.citation.title | ACS Synthetic Biology | - |
dc.citation.volume | 5 | - |
dc.contributor.affiliatedAuthor | Noh, MH | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-85017356007 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 24 | - |
dc.description.scptc | 16 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | N-BUTANOL | - |
dc.subject.keywordPlus | MEVALONATE PATHWAY | - |
dc.subject.keywordPlus | HIGH-THROUGHPUT | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | 1,3-PROPANEDIOL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordAuthor | synthetic biology | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordAuthor | balancing enzyme activity | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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