DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gootenberg, Jonathan S. | - |
dc.contributor.author | Abudayyeh, Omar O. | - |
dc.contributor.author | Lee, Jeong Wook | - |
dc.contributor.author | Essletzbichler, Patrick | - |
dc.contributor.author | Dy, Aaron J. | - |
dc.contributor.author | Joung, Julia | - |
dc.contributor.author | Verdine, Vanessa | - |
dc.contributor.author | Donghia, Nina | - |
dc.contributor.author | Daringer, Nicole M. | - |
dc.contributor.author | Freije, Catherine A. | - |
dc.contributor.author | Myhrvold, Cameron | - |
dc.contributor.author | Bhattacharyya, Roby P. | - |
dc.contributor.author | Livny, Jonathan | - |
dc.contributor.author | Regev, Aviv | - |
dc.contributor.author | Koonin, Eugene V. | - |
dc.contributor.author | Hung, Deborah T. | - |
dc.contributor.author | Sabeti, Pardis C. | - |
dc.contributor.author | Collins, James J. | - |
dc.contributor.author | Zhang, Feng | - |
dc.date.accessioned | 2018-01-04T10:36:29Z | - |
dc.date.available | 2018-01-04T10:36:29Z | - |
dc.date.created | 2017-09-26 | - |
dc.date.issued | 2017-04-28 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/39148 | - |
dc.description.abstract | Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumor DNA. Furthermore, SHERLOCK reaction reagents can be lyophilized for cold-chain independence and long-term storage and be readily reconstituted on paper for field applications. | - |
dc.language | English | - |
dc.publisher | AAAS | - |
dc.relation.isPartOf | Science | - |
dc.title | Nucleic acid detection with CRISPR-Cas13a/C2c2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1126/science.aam9321 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Science, v.356, no.6336, pp.438 - 442 | - |
dc.identifier.wosid | 000400143000054 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 442 | - |
dc.citation.number | 6336 | - |
dc.citation.startPage | 438 | - |
dc.citation.title | Science | - |
dc.citation.volume | 356 | - |
dc.contributor.affiliatedAuthor | Lee, Jeong Wook | - |
dc.identifier.scopusid | 2-s2.0-85017652697 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 137 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | TIO2 | - |
dc.subject.keywordPlus | ATOMIC LAYER DEPOSITION | - |
dc.subject.keywordPlus | LIGHT-EMITTING-DIODES | - |
dc.subject.keywordPlus | SOLAR-CELLS | - |
dc.subject.keywordPlus | HALIDE PEROVSKITES | - |
dc.subject.keywordPlus | LEAD IODIDE | - |
dc.subject.keywordPlus | ZINC-OXIDE | - |
dc.subject.keywordPlus | AL2O3 | - |
dc.subject.keywordPlus | DEVICES | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.