Altered branching patterns of Purkinje cells in mouse model for cortical development disorder
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SCOPUS
- Title
- Altered branching patterns of Purkinje cells in mouse model for cortical development disorder
- Authors
- Jinkyung Kim; Namseop Kwon; Soeun Chang; KIM, KYONG TAI; Dongmyeong Lee; KIM, SEUNGHWAN; So Jeong Yun; HWANG, DAEHEE; Jee Woong Kim; Yeukuang Hwu; Giorgio Margaritondo; JE, JUNG HO; Im Joo Rhyu
- Date Issued
- 2011-10-18
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reeler, we visualized in 3-D geometry the shape alterations of planar PC dendrites (i.e., abnormal 3-D arborization). Despite these alterations, the 3-D quantitative analysis of the branching patterns showed no significant changes of the 77 +/- 8 degrees branch angle, whereas the branch segment length strongly increased with large fluctuations, comparing to control. The 3-D fractal dimension of the PCs decreased from 1.723 to 1.254, indicating a significant reduction of dendritic complexity. This study provides insights into etiologies and further potential treatment options for lissencephaly and various neurodevelopmental disorders.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/41371
- DOI
- 10.1038/SREP00122
- ISSN
- 2045-2322
- Article Type
- Article
- Citation
- Scientific Reports, vol. 1, page. 122, 2011-10-18
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