Ripply-mediated Xbra inhibition is required for proper head development
- Title
- Ripply-mediated Xbra inhibition is required for proper head development
- Authors
- HAN, JIN KWAN; YEO, IN CHUL
- Date Issued
- 2017-05-10
- Publisher
- JSDB
- Abstract
- Spemann ’ s Organizer induces dorsal and anterior fate to nearby
tissues and make head and body axis in Xenopus embryos. Various
molecules have been discovered to mediate this organizing activity.
We found Ripply as a noble organizer gene through microarray
analysis. Ripply has been reported as a somitogenesis regulating
factor. Ripply binds to Tbx6 and transforms Tbx6 to a transcriptional
repressor in developing somites by recruiting Groucho/TLE corepressors.
However, Ripply was also expressed in dorsal
mesendoderm of early gastrula and in head organizer of late
gastrula. Ectopic expression of Ripply induced blastopore closure
defects and neural tube closure defects, while knockdown of Ripply
induced truncated head. In molecular level, Ripply interacted with
Xbra, and inhibited the target genes of Xbra. Ripply knockdown
resulted in the reduction of head-related genes such as Goosecoid
and Otx2. These result suggest that Ripply maintains the fate of the
head mesoderm by inhibiting Xbra, and this function is necessary for
proper head development.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/42212
- Article Type
- Conference
- Citation
- 50TH ANNUAL MEETING OF THE JSDB 2017, 2017-05-10
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