DC Field | Value | Language |
---|---|---|
dc.contributor.author | Su, Dongdong | - |
dc.contributor.author | Chai Lean Teoh | - |
dc.contributor.author | Sahu, Srikanta | - |
dc.contributor.author | Das, Raj Kumar | - |
dc.contributor.author | Chang, Young-Tae | - |
dc.date.accessioned | 2018-06-15T05:16:37Z | - |
dc.date.available | 2018-06-15T05:16:37Z | - |
dc.date.created | 2017-09-08 | - |
dc.date.issued | 2014-07 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/50292 | - |
dc.description.abstract | We designed a red-emitting turn-on FRET-based molecular probe 1 for selective detection of cysteine and homocysteine. Probe 1 shows significant fluorescence enhancement after cleavage of the 2, 4-dinitrobenzensulfonyl (DNBS) unit from the fluorophore upon thiols treatment. The precursor of probe 1, BNM153, is a moderate quantum yield FRET dye which contributes a minimum emission leakage from its donor part. We synthesized this assembly by connecting a low quantum yield (less than 1%) BODIPY donor to a high quantum yield BODIPY acceptor via a 1, 3-triazine bridge system. It is noteworthy that the majority of the non-radiative energy loss of donor (BDN) was converted to the acceptor (BDM)'s fluorescence output with minimum leaks of donor emission. The fluorescence sensing mechanism of probe 1 was illustrated by fluorescence spectroscopy, kinetic measurements, HPLC-MS analysis and DFT calculations. Probe 1 is pH-independent at the physiological pH range. Finally, live cells imaging demonstrated the utility of probe 1 as a biosensor for thiols. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.subject | SELECTIVE FLUORESCENT-PROBE | - |
dc.subject | LIVING CELLS | - |
dc.subject | CYSTEINE | - |
dc.subject | GLUTATHIONE | - |
dc.subject | HOMOCYSTEINE | - |
dc.subject | SENSOR | - |
dc.subject | LUMINESCENT | - |
dc.subject | CHEMODOSIMETER | - |
dc.subject | EMISSION | - |
dc.subject | DESIGN | - |
dc.title | Live cells imaging using a turn-on FRET-based BODIPY probe for biothiols | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.biomaterials.2014.04.035 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.35, no.23, pp.6078 - 6085 | - |
dc.identifier.wosid | 000337212200010 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 6085 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 6078 | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 35 | - |
dc.contributor.affiliatedAuthor | Chang, Young-Tae | - |
dc.identifier.scopusid | 2-s2.0-84900018885 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 44 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SELECTIVE FLUORESCENT-PROBE | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | CYSTEINE | - |
dc.subject.keywordPlus | GLUTATHIONE | - |
dc.subject.keywordPlus | HOMOCYSTEINE | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | LUMINESCENT | - |
dc.subject.keywordPlus | CHEMODOSIMETER | - |
dc.subject.keywordPlus | EMISSION | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordAuthor | Fluorescence | - |
dc.subject.keywordAuthor | Fluorescence resonance energy transfer (FRET) | - |
dc.subject.keywordAuthor | Biothiols | - |
dc.subject.keywordAuthor | cysteine | - |
dc.subject.keywordAuthor | Sensor | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
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