Hyaluronate-Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer
SCIE
SCOPUS
- Title
- Hyaluronate-Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer
- Authors
- Beack, S.; Cho, M.; Kim, Y.-E.; Ahn, G.-O.; Hahn, S.K.
- Date Issued
- 2017-03
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Colon cancer is one of the most common death-related cancers in the world. For treating colon cancer, it is crucial to detect and remove malignant lesions early. Here, we developed hyaluronate (HA)-peanut agglutinin (PNA) conjugates for the bioimaging of colon cancer. The HA-PNA conjugates were successfully synthesized by the coupling reaction between aldehyde-modified HA and the N-terminal amine group of PNA. For diagnostic imaging, rhodamine B (RhoB) was chemically conjugated onto PNA in HA-PNA conjugates. After intraluminal injection of HA-PNA-RhoB conjugates into tumor-bearing mice, small-sized colon cancers could be effectively visualized by ex vivo imaging with an in vivo imaging system (IVIS) and a two-photon microscope. With these results taken together, we could confirm the feasibility of HA-PNA-RhoB conjugates as a bioimaging agent for detecting colon cancers. ? 2017 American Chemical Society.
- Keywords
- aldehyde; amine; contrast medium; hyaluronic acid; peanut agglutinin; rhodamine B; dextran sulfate; hyaluronic acid; peanut agglutinin; animal experiment; animal model; Article; bioimaging; cancer diagnosis; chemical reaction; chemical structure; colon cancer; conjugate; cytotoxicity test; diagnostic imaging; fluorescence microscopy; gel permeation chromatography; HCT 116 cell line; human; human cell; human cell culture; in vitro study; intraluminal drug administration; mouse; nonhuman; polyacrylamide gel electrophoresis; synthesis; animal; C57BL mouse; cell proliferation; chemically induced; chemistry; colon tumor; drug effects; drug screening; fluorescence microscopy; male; metabolism; pathology; procedures; tissue distribution; tumor cell culture; Animals; Cell Proliferation; Colonic Neoplasms; Dextran Sulfate; Humans; Hyaluronic Acid; Male; Mice; Mice, Inbred C57BL; Microscopy, Fluorescence; Peanut Agglutinin; Tissue Distribution; Tumor Cells, Cultured; Xenograft Model Antitumor Assays
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50692
- DOI
- 10.1021/acs.bioconjchem.7b00126
- ISSN
- 1043-1802
- Article Type
- Article
- Citation
- BIOCONJUGATE CHEMISTRY, vol. 28, no. 5, page. 1434 - 1442, 2017-03
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- There are no files associated with this item.
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