DC Field | Value | Language |
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dc.contributor.author | Lee, M.H. | - |
dc.contributor.author | Yoo, Y.-J. | - |
dc.contributor.author | Kim, D.H. | - |
dc.contributor.author | Hanh, N.H. | - |
dc.contributor.author | Kwon, Y. | - |
dc.contributor.author | Hwang, I. | - |
dc.date.accessioned | 2018-06-15T05:56:17Z | - |
dc.date.available | 2018-06-15T05:56:17Z | - |
dc.date.created | 2017-12-21 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/51002 | - |
dc.description.abstract | Prenylated Rab acceptor1 (PRA1) functions in the recruitment of prenylated Rab proteins to their cognate organelles. Arabidopsis (Arabidopsis thaliana) contains a large number of proteins belonging to the AtPRA1 family. However, their physiological roles remain largely unknown. Here, we investigated the physiological role of AtPRA1.F4, a member of the AtPRA1 family. A T-DNA insertion knockdown mutant of AtPRA1.F4, atpra1.f4, was smaller in stature than parent plants and possessed shorter roots, whereas transgenic plants overexpressing HA:AtPRA1.F4 showed enhanced development of secondary roots and root hairs. However, both overexpression and knockdown plants exhibited increased sensitivity to high-salt stress, lower vacuolar Na+/K+-ATPase and plasma membrane ATPase activities, lower and higher pH in the vacuole and apoplast, respectively, and highly vesiculated Golgi apparatus. HA:AtPRA1.F4 localized to the Golgi apparatus and assembled into high-molecular-weight complexes. atpra1.f4 plants displayed a defect in vacuolar trafficking, which was complemented by low but not high levels of HA:AtPRA1.F4. Overexpression of HA:AtPRA1.F4 also inhibited protein trafficking at the Golgi apparatus, albeit differentially depending on the final destination or type of protein: trafficking of vacuolar proteins, plasma membrane proteins, and trans-Golgi network (TGN)-localized SYP61 was strongly inhibited; trafficking of TGN-localized SYP51 was slightly inhibited; and trafficking of secretory proteins and TGN-localized SYP41 was negligibly or not significantly inhibited. Based on these results, we propose that Golgi-localized AtPRA1.F4 is involved in the exit of many but not all types of post-Golgi proteins from the Golgi apparatus. Additionally, an appropriate level of AtPRA1.F4 is crucial for its function at the Golgi apparatus. ? 2017 American Society of Plant Biologists. All rights reserved. | - |
dc.language | English | - |
dc.publisher | American Society of Plant Biologists | - |
dc.relation.isPartOf | Plant Physiology | - |
dc.title | The prenylated rab GTPase receptor PRA1.F4 contributes to protein exit from the golgi apparatus | - |
dc.type | Article | - |
dc.identifier.doi | 10.1104/pp.17.00466 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Plant Physiology, v.174, no.3, pp.1576 - 1594 | - |
dc.identifier.wosid | 000404233000022 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1594 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1576 | - |
dc.citation.title | Plant Physiology | - |
dc.citation.volume | 174 | - |
dc.contributor.affiliatedAuthor | Hwang, I. | - |
dc.identifier.scopusid | 2-s2.0-85021786506 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | DISTINCT MEMBRANE COMPARTMENTS | - |
dc.subject.keywordPlus | YEAST SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | PLANT-CELLS | - |
dc.subject.keywordPlus | VACUOLAR TRAFFICKING | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | INTRACELLULAR TRAFFICKING | - |
dc.subject.keywordPlus | PREVACUOLAR COMPARTMENT | - |
dc.subject.keywordPlus | VESICULAR TRANSPORT | - |
dc.subject.keywordPlus | ENDOMEMBRANE SYSTEM | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
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