Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes
SCIE
SCOPUS
- Title
- Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes
- Authors
- Itoh, Hideki; Arai, Satoshi; Sudhaharan, Thankiah; Lee, Sung-Chan; Chang, Young-Tae; Ishiwata, Shin'ichi; Suzuki, Madoka; Lane, E. Birgitte
- Date Issued
- 2016-01
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.
- Keywords
- SKELETAL-MUSCLE CELLS; 10(5) GAP ISSUE; LIVING CELLS; RYANODINE RECEPTOR; ENDOPLASMIC-RETICULUM; TEMPERATURE; THERMOGENESIS; THERMOSENSORS; RELEASE; HEAT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/51034
- DOI
- 10.1039/c5cc09943a
- ISSN
- 1359-7345
- Article Type
- Article
- Citation
- CHEMICAL COMMUNICATIONS, vol. 52, no. 24, page. 4458 - 4461, 2016-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.