DC Field | Value | Language |
---|---|---|
dc.contributor.author | 공지현 | en_US |
dc.date.accessioned | 2014-12-01T11:46:16Z | - |
dc.date.available | 2014-12-01T11:46:16Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.other | OAK-2014-00065 | en_US |
dc.identifier.uri | http://postech.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000000546338 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/567 | - |
dc.description | Master | en_US |
dc.description.abstract | Despite clinical exploitation of exendin 4 for the treatment of type 2 diabetes, the significantly short half-life requiring twice a day injection has limited the wide applications. In this work, a novel protocol for the synthesis of long acting hyaluronate (HA) ? exendin 4 conjugate was successfully developed using Michael addition chemistry between vinyl sulfone modified HA (HA-VS) and thiolated exendin 4. The exendin 4 content could be controlled in the range of 5~30 molecules per single HA chain with a bioconjugation efficiency higher than 90%. The conjugation of exendin 4 with HA resulted in about 20 times improved in vitro serum stability maintaining the hypoglycemic and gluco regulatory bioactivities of exendin 4. HA ? exendin 4 conjugates showed excellent glucose-lowering capabilities in type 2 db/db mice demonstrating protracted hypoglycemic effect up to 3 days after a single subcutaneous injection. Furthermore, insulin immunohistochemical analysis of islets in db/db mice confirmed the improved insulinotropic activity of HA ? exendin 4 conjugates. The novel HA ? exendin 4 conjugates will be investigated further as a twice a week injection dosage form for clinical applications. | en_US |
dc.language | eng | en_US |
dc.publisher | 포항공과대학교 | en_US |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | Hyaluronic Acid Based Drug Delivery System for the Treatment of Type 2 Diabetes | en_US |
dc.type | Thesis | en_US |
dc.contributor.college | 일반대학원 신소재공학과 | en_US |
dc.date.degree | 2010- 2 | en_US |
dc.type.docType | Thesis | - |
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