DC Field | Value | Language |
---|---|---|
dc.contributor.author | Masuda, T. | - |
dc.contributor.author | Suzuki, M. | - |
dc.contributor.author | Inoue, S. | - |
dc.contributor.author | Song, C. | - |
dc.contributor.author | Nakane, T. | - |
dc.contributor.author | Nango, E. | - |
dc.contributor.author | Tanaka, R. | - |
dc.contributor.author | Tono, K. | - |
dc.contributor.author | Joti, Y. | - |
dc.contributor.author | Kameshima, T. | - |
dc.contributor.author | Hatsui, T. | - |
dc.contributor.author | Yabashi, M. | - |
dc.contributor.author | Mikami, B. | - |
dc.contributor.author | Nureki, O. | - |
dc.contributor.author | Numata, K. | - |
dc.contributor.author | Iwata, S. | - |
dc.contributor.author | Sugahara, M. | - |
dc.date.accessioned | 2018-07-17T10:47:10Z | - |
dc.date.available | 2018-07-17T10:47:10Z | - |
dc.date.created | 2017-12-21 | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/92124 | - |
dc.description.abstract | Atomic resolution structures (beyond 1.20 ?) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes. Serial femtosecond crystallography (SFX) has attracted surging interest by providing a route to bypass such challenges. Yet the progress on atomic resolution analysis with SFX has been rather slow. In this report, we describe the 1.20 ? resolution structure of proteinase K using 13 keV photon energy. Hydrogen atoms, water molecules, and a number of alternative side-chain conformations have been resolved. The increase in the value of B-factor in SFX suggests that the residues and water molecules adjacent to active sites were flexible and exhibited dynamic motions at specific substrate-recognition sites. ? 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | Scientific Reports | - |
dc.title | Atomic resolution structure of serine protease proteinase K at ambient temperature | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/srep45604 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.7 | - |
dc.identifier.wosid | 000397960800001 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 7 | - |
dc.contributor.affiliatedAuthor | Song, C. | - |
dc.identifier.scopusid | 2-s2.0-85016545753 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 4 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SYNCHROTRON-RADIATION | - |
dc.subject.keywordPlus | ACTIVE-SITE | - |
dc.subject.keywordPlus | DATA QUALITY | - |
dc.subject.keywordPlus | FREE-ELECTRON LASER | - |
dc.subject.keywordPlus | TRITIRACHIUM-ALBUM-LIMBER | - |
dc.subject.keywordPlus | X-RAY-DIFFRACTION | - |
dc.subject.keywordPlus | BARRIER HYDROGEN-BOND | - |
dc.subject.keywordPlus | ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | FEMTOSECOND CRYSTALLOGRAPHY | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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