DC Field | Value | Language |
---|---|---|
dc.contributor.author | Na, J | - |
dc.contributor.author | Shin, GW | - |
dc.contributor.author | Jung, GY | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2015-06-25T01:01:07Z | - |
dc.date.available | 2015-06-25T01:01:07Z | - |
dc.date.created | 2014-03-04 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.other | 2015-OAK-0000029109 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/9328 | - |
dc.description.abstract | Aberrant DNA methylation is a potential diagnostic marker for complex diseases, such as cancer. With the increase in the number of genes known to exhibit disease-associated aberrant methylation, the need for accurate multiplex assays for quantifying DNA methylation has increased. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is one method that has been highlighted in this context. However, two limitations make the custom design of MS-MLPA assays impractical: the need for long probes containing stuffer sequences and a reliance on only one restriction enzyme. Here, we developed a variation of MS-MLPA that employs a simpler probe-design process. To overcome the above-mentioned limitations, we used stuffer-free MS-MLPA probes that are subsequently analyzed using high-resolution capillary electrophoresis-based single-strand conformational polymorphism (CE-SSCP) instead of conventional length-dependent CE. Moreover, multiple methylation-sensitive restriction enzymes (HhaI, HpaII, and AciI) were used simultaneously; thus, probes satisfying desired criteria were available for all targets. Using this assay concept, we analyzed 17 genes associated with hepatocellular carcinoma. Our results showed that the custom-designed assay based on MS-MLPA-CE-SSCP provided robust multiplex quantification of DNA methylation levels. | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | Analyst | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | A robust and simple-to-design multiplex DNA methylation assay based on MS-MLPA-CE-SSCP | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | en_US |
dc.identifier.doi | 10.1039/C3AN01178J | - |
dc.author.google | Na, J | en_US |
dc.author.google | Shin, GW | en_US |
dc.author.google | Jung, GY | en_US |
dc.relation.volume | 138 | en_US |
dc.relation.issue | 22 | en_US |
dc.relation.startpage | 6969 | en_US |
dc.relation.lastpage | 6976 | en_US |
dc.contributor.id | 10130678 | en_US |
dc.relation.journal | Analyst | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Analyst, v.138, no.22, pp.6969 - 6976 | - |
dc.identifier.wosid | 000325819300034 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 6976 | - |
dc.citation.number | 22 | - |
dc.citation.startPage | 6969 | - |
dc.citation.title | Analyst | - |
dc.citation.volume | 138 | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-84885947261 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 6 | - |
dc.description.scptc | 6 | * |
dc.date.scptcdate | 2018-10-274 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | DEPENDENT PROBE AMPLIFICATION | - |
dc.subject.keywordPlus | CPG ISLANDS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | ELECTROPHORESIS | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | PATTERNS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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