DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YS | - |
dc.contributor.author | Cha, HJ | - |
dc.date.accessioned | 2015-06-25T01:02:11Z | - |
dc.date.available | 2015-06-25T01:02:11Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2006-10 | - |
dc.identifier.issn | 0066-4804 | - |
dc.identifier.other | 2015-OAK-0000010699 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/9343 | - |
dc.description.abstract | Amphipathic antimicrobial peptides can destroy bacteria cells by inducing membrane permeabilization, forming one strategy for innate defense by various organisms. However, although the antimicrobial peptides are considered a promising alternative for use against multidrug-resistant bacteria, large-scale screening of potential candidate antimicrobial peptides will require a simple, rapid assay for antimicrobial activity. Here, we describe a novel fluorescence resonance energy transfer (FRET)-based assay system for antimicrobial peptides which takes advantage of pH-related changes in FRET efficiency due to the instability of enhanced yellow fluorescent protein versus the stability of enhanced cyan fluorescent protein in a reduced-pH environment. We successfully showed that quantification of antimicrobial activity is possible through a difference of FRET efficiency between ECFP-EYFP fusion molecules released from disrupted Escherichia coli in an extracellular environment (pH 6) and those retained in an intracellular environment (pH similar to 7). Thus, we herein suggest a new simple, effective, and efficient pH-controlled FRET-based antimicrobial peptide screening method applicable to high-throughput screening of candidate peptide libraries. | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.relation.isPartOf | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | High-throughput and facile assay of antimicrobial peptides using pH-controlled fluorescence resonance energy transfer | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | en_US |
dc.identifier.doi | 10.1128/AAC.00455-06 | - |
dc.author.google | Kim, YS | en_US |
dc.author.google | Cha, HJ | en_US |
dc.relation.volume | 50 | en_US |
dc.relation.issue | 10 | en_US |
dc.relation.startpage | 3330 | en_US |
dc.relation.lastpage | 3335 | en_US |
dc.contributor.id | 10057405 | en_US |
dc.relation.journal | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, v.50, no.10, pp.3330 - 3335 | - |
dc.identifier.wosid | 000241021700015 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 3335 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 3330 | - |
dc.citation.title | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | - |
dc.citation.volume | 50 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-33749536928 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 10 | - |
dc.description.scptc | 10 | * |
dc.date.scptcdate | 2018-10-274 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | COMBINATORIAL LIBRARIES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | GENERATION | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
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