DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tae Wu Kim | - |
dc.contributor.author | Jae Hyuk Lee | - |
dc.contributor.author | Jungkweon Choi | - |
dc.contributor.author | KIM, KYUNG HWAN | - |
dc.contributor.author | Luuk J. van Wilderen | - |
dc.contributor.author | Laurent Guerin | - |
dc.contributor.author | Youngmin Kim | - |
dc.contributor.author | Yang Ouk Jung | - |
dc.contributor.author | Cheolhee Yang | - |
dc.contributor.author | Jeongho Kim | - |
dc.contributor.author | Michael Wulff | - |
dc.contributor.author | Jasper J. van Thor | - |
dc.contributor.author | Hyotcherl Ihee | - |
dc.date.accessioned | 2018-11-12T01:47:14Z | - |
dc.date.available | 2018-11-12T01:47:14Z | - |
dc.date.created | 2018-10-24 | - |
dc.date.issued | 2012-02-15 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/94155 | - |
dc.description.abstract | Photoreceptor proteins play crucial roles in receiving light stimuli that give rise to the responses required for biological function. However, structural characterization of conformational transition of the photoreceptors has been elusive in their native aqueous environment, even for a prototype photoreceptor, photoactive yellow protein (PYP). We employ pump probe X-ray solution scattering to probe the structural changes that occur during the photocycle of PYP in a wide time range from 3.16 mu s to 300 ms. By the analysis of both kinetics and structures of the intermediates, the structural progression of the protein in the solution phase is vividly visualized. We identify four structurally distinct intermediates and their associated five time constants and reconstructed the molecular shapes of the four intermediates from time-independent, species-associated difference scattering curves. The constructed structures of the intermediates show the large conformational changes such as the protrusion of N-terminus, which is restricted in the crystalline phase due to the crystal contact and thus could not be clearly observed by X-ray crystallography. The protrusion of the N-terminus and the protein volume gradually increase with the progress of the photocycle and becomes maximal in the final intermediate, which is proposed to be the signaling state. The data not only reveal that a common kinetic mechanism is applicable to both the crystalline and the solution phases, but also provide direct evidence for how the sample environment influences structural dynamics and the reaction rates of the PYP photocycle. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.title | Protein Structural Dynamics of Photoactive Yellow Protein in Solution Revealed by Pump–Probe X-ray Solution Scattering | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/ja210435n | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.134, no.6, pp.3145 - 3153 | - |
dc.identifier.wosid | 000301161500055 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 3153 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 3145 | - |
dc.citation.title | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.citation.volume | 134 | - |
dc.contributor.affiliatedAuthor | KIM, KYUNG HWAN | - |
dc.identifier.scopusid | 2-s2.0-84863168385 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 71 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SMALL-ANGLE SCATTERING | - |
dc.subject.keywordPlus | BIOLOGICAL MACROMOLECULES | - |
dc.subject.keywordPlus | SIGNALING STATE | - |
dc.subject.keywordPlus | IONIC-STRENGTH | - |
dc.subject.keywordPlus | PHOTOCYCLE | - |
dc.subject.keywordPlus | INTERMEDIATE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | CRYSTALLOGRAPHY | - |
dc.subject.keywordPlus | LANDSCAPE | - |
dc.subject.keywordPlus | I-1 | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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