Design and optimization of genetically encoded biosensors for high-throughput screening of chemicals
SCIE
SCOPUS
- Title
- Design and optimization of genetically encoded biosensors for high-throughput screening of chemicals
- Authors
- LIM, HYUNGYU; JANG, SUNGHO; SUNGYEON, JANG; Seo, Sang Woo; Jung, Gyoo Yeol
- Date Issued
- 2018-12
- Publisher
- CURRENT BIOLOGY LTD
- Abstract
- Evolutionary engineering of microbes for the production of metabolites requires efficient screening methods to test vast mutant libraries. Genetically encoded biosensors are regarded as promising screening devices owing to their wide range of detectable ligands and great applicability to high-throughput screening and selection. Here, we reviewed the current progress in design and optimization of biosensors for high throughput screening of chemicals. First, we summarized genetic parts of biosensors and strategies for their discovery and development. Next, we explained the properties of biosensors that are relevant to high-throughput screening. Finally, we described various methods for tuning biosensors to fulfill requirements of an efficient screening.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94481
- DOI
- 10.1016/j.copbio.2018.01.011
- ISSN
- 0958-1669
- Article Type
- Article
- Citation
- CURRENT OPINION IN BIOTECHNOLOGY, vol. 54, page. 18 - 25, 2018-12
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- There are no files associated with this item.
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