DC Field | Value | Language |
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dc.contributor.author | Seok, Joo Yeon | - |
dc.contributor.author | Yang, Jina | - |
dc.contributor.author | Choi, Sang Jin | - |
dc.contributor.author | Lim, Hyun Gyu | - |
dc.contributor.author | Choi, Un Jong | - |
dc.contributor.author | Kim, Kyung-Jin | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Yoo, Tae Hyeon | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2018-12-13T07:44:05Z | - |
dc.date.available | 2018-12-13T07:44:05Z | - |
dc.date.created | 2018-06-22 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/94522 | - |
dc.description.abstract | 3-Hydroxypropionic acid (3-HP) is an important platform chemical, and biological production of 3-HP from glycerol as a carbon source using glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) has been revealed to be effective because it involves a relatively simple metabolic pathway and exhibits higher yield and productivity than other biosynthetic pathways. Despite the successful attempts of 3-HP production from glycerol, the biological process suffers from problems arising from low activity and inactivation of the two enzymes. To apply the directed evolutionary approach to engineer the 3-HP production system, we constructed a synthetic selection device using a 3-HP-responsive transcription factor and developed a selection approach for screening 3-HP-producing microorganisms. The method was applied to an ALDH library, specifically aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). Only two serial cultures resulted in enrichment of strains showing increased 3-HP production, and an isolated KGSADH variant enzyme exhibited a 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild-type one. This approach will provide the simple and efficient tool to engineer the pathway enzymes in metabolic engineering. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | METABOLIC ENGINEERING | - |
dc.title | Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using a synthetic selection device | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ymben.2018.03.009 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.47, pp.113 - 120 | - |
dc.identifier.wosid | 000433423600012 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 120 | - |
dc.citation.startPage | 113 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 47 | - |
dc.contributor.affiliatedAuthor | Seok, Joo Yeon | - |
dc.contributor.affiliatedAuthor | Lim, Hyun Gyu | - |
dc.contributor.affiliatedAuthor | Jung, Gyoo Yeol | - |
dc.identifier.scopusid | 2-s2.0-85044109129 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 4 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordAuthor | Directed evolution | - |
dc.subject.keywordAuthor | Transcription factor | - |
dc.subject.keywordAuthor | Screening | - |
dc.subject.keywordAuthor | Synthetic biology | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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