DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, NK | - |
dc.contributor.author | Kapanidis, AN | - |
dc.contributor.author | Koh, HR | - |
dc.contributor.author | Korlann, Y | - |
dc.contributor.author | Ho, SO | - |
dc.contributor.author | Kim, Y | - |
dc.contributor.author | Gassman, N | - |
dc.contributor.author | Kim, SK | - |
dc.contributor.author | Weiss, S | - |
dc.date.accessioned | 2015-06-25T01:34:03Z | - |
dc.date.available | 2015-06-25T01:34:03Z | - |
dc.date.created | 2009-09-30 | - |
dc.date.issued | 2007-01 | - |
dc.identifier.issn | 0006-3495 | - |
dc.identifier.other | 2015-OAK-0000018164 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/9843 | - |
dc.description.abstract | We introduce three-color alternating-laser excitation (3c-ALEX), a fluorescence resonance energy transfer (FRET) method that measures up to three intramolecular distances and complex interaction stoichiometries of single molecules in solution. This tool extends substantially the capabilities of two-color ALEX, which employs two alternating lasers to study molecular interactions (through probe stoichiometry S) and intramolecular distances (through FRET efficiency E), and sorts fluorescent molecules in multi-dimensional probe-stoichiometry and FRET-efficiency histograms. Probe-stoichiometry histograms allowed analytical sorting, identification, and selection of diffusing species; selected molecules were subsequently represented in FRET-efficiency histograms, generating up to three intramolecular distances. Using triply labeled DNAs, we established that 3c-ALEX enables 1), FRET-independent analysis of three-component interactions; 2), observation and sorting of singly, doubly, and triply labeled molecules simultaneously present in solution; 3), measurements of three intramolecular distances within single molecules from a single measurement; and 4), dissection of conformational heterogeneity with improved resolution compared to conventional single-molecule FRET. We also used 3c-ALEX to study large biomolecules such as RNA polymerase-DNA transcription complexes, and monitor the downstream translocation of RNA polymerase on DNA from two perspectives within the complex. This study paves the way for advanced single-molecule analysis of complex mixtures and biomolecular machinery. | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | BIOPHYSICAL SOCIETY | - |
dc.relation.isPartOf | BIOPHYSICAL JOURNAL | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | THREE-COLOR ALTERNATING-LASER EXCITATION OF SINGLE MOLECULES: MONITORING MULTIPLE INTERACTIONS AND DISTANCES | - |
dc.type | Article | - |
dc.contributor.college | 시스템생명공학부 | en_US |
dc.identifier.doi | 10.1529/BIOPHYSJ.106 | - |
dc.author.google | Lee, NK | en_US |
dc.author.google | Kapanidis, AN | en_US |
dc.author.google | Weiss, S | en_US |
dc.author.google | Kim, SK | en_US |
dc.author.google | Gassman, N | en_US |
dc.author.google | Kim, Y | en_US |
dc.author.google | Ho, SO | en_US |
dc.author.google | Korlann, Y | en_US |
dc.author.google | Koh, HR | en_US |
dc.relation.volume | 92 | en_US |
dc.relation.issue | 1 | en_US |
dc.relation.startpage | 303 | en_US |
dc.relation.lastpage | 312 | en_US |
dc.contributor.id | 10206847 | en_US |
dc.relation.journal | BIOPHYSICAL JOURNAL | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOPHYSICAL JOURNAL, v.92, no.1, pp.303 - 312 | - |
dc.identifier.wosid | 000242754300036 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 312 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 303 | - |
dc.citation.title | BIOPHYSICAL JOURNAL | - |
dc.citation.volume | 92 | - |
dc.contributor.affiliatedAuthor | Lee, NK | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 127 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | RNA-POLYMERASE | - |
dc.subject.keywordPlus | FLUORESCENCE SPECTROSCOPY | - |
dc.subject.keywordPlus | CONFORMATIONAL DYNAMICS | - |
dc.subject.keywordPlus | SIGMA(70) SUBUNIT | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DNA-BINDING | - |
dc.subject.keywordPlus | FRET | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
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