DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Julianna | - |
dc.contributor.author | Perez, Jasmine T. | - |
dc.contributor.author | Chen, Cindy | - |
dc.contributor.author | Li, Yan | - |
dc.contributor.author | Benitez, Asiel | - |
dc.contributor.author | Kandasamy, Matheswaran | - |
dc.contributor.author | Lee, Yoontae | - |
dc.contributor.author | Andrade, Jorge | - |
dc.contributor.author | tenoever, Benjamin | - |
dc.contributor.author | Manicassamy, Balaji | - |
dc.date.accessioned | 2019-07-04T10:10:03Z | - |
dc.date.available | 2019-07-04T10:10:03Z | - |
dc.date.created | 2018-07-20 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/99297 | - |
dc.description.abstract | The emergence of influenza A viruses (IAVs) from zoonotic reservoirs poses a great threat to human health. As seasonal vaccines are ineffective against zoonotic strains, and newly transmitted viruses can quickly acquire drug resistance, there remains a need for host-directed therapeutics against IAVs. Here, we performed a genome-scale CRISPR/Cas9 knockout screen in human lung epithelial cells with a human isolate of an avian H5N1 strain. Several genes involved in sialic acid biosynthesis and related glycosylation pathways were highly enriched post-H5N1 selection, including SLC35A1, a sialic acid transporter essential for IAV receptor expression and thus viral entry. Importantly, we have identified capicua (CIC) as a negative regulator of cell-intrinsic immunity, as loss of CIC resulted in heightened antiviral responses and restricted replication of multiple viruses. Therefore, our study demonstrates that the CRISPR/Cas9 system can be utilized for the discovery of host factors critical for the replication of intracellular pathogens. | - |
dc.language | English | - |
dc.publisher | Cell Press | - |
dc.relation.isPartOf | Cell Reports | - |
dc.title | Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.celrep.2018.03.045 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Cell Reports, v.23, no.2, pp.596 - 607 | - |
dc.identifier.wosid | 000429866600022 | - |
dc.citation.endPage | 607 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 596 | - |
dc.citation.title | Cell Reports | - |
dc.citation.volume | 23 | - |
dc.contributor.affiliatedAuthor | Lee, Yoontae | - |
dc.identifier.scopusid | 2-s2.0-85045214365 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | WEST-NILE-VIRUS | - |
dc.subject.keywordPlus | A VIRUS | - |
dc.subject.keywordPlus | DEPENDENCY FACTORS | - |
dc.subject.keywordPlus | DENGUE VIRUS | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | RNAI | - |
dc.subject.keywordPlus | ENTRY | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | CAPICUA | - |
dc.subject.keywordPlus | INFECTION | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
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