DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gao, M. | - |
dc.contributor.author | KIM, YOUNGKI | - |
dc.contributor.author | Zhang, C. | - |
dc.contributor.author | Borshch, V. | - |
dc.contributor.author | Zhou, S. | - |
dc.contributor.author | Park, H. -S. | - |
dc.contributor.author | Jakli, A. | - |
dc.contributor.author | Lavrentovich, O. D. | - |
dc.contributor.author | Tamba, M. -G. | - |
dc.contributor.author | Kohlmeier, A. | - |
dc.contributor.author | Mehl, G. H. | - |
dc.contributor.author | Weissflog, W. | - |
dc.contributor.author | Studer, D. | - |
dc.contributor.author | Zuber, B. | - |
dc.contributor.author | Gnagi, H. | - |
dc.contributor.author | Lin, F. | - |
dc.date.accessioned | 2019-08-07T02:10:16Z | - |
dc.date.available | 2019-08-07T02:10:16Z | - |
dc.date.created | 2019-07-26 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.issn | 1097-0029 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/99502 | - |
dc.description.abstract | Liquid crystals (LCs) represent a challenging group of materials for direct transmission electron microscopy (TEM) studies due to the complications in specimen preparation and the severe radiation damage. In this paper, we summarize a series of specimen preparation methods, including thin film and cryo-sectioning approaches, as a comprehensive toolset enabling high-resolution direct cryo-TEM observation of a broad range of LCs. We also present comparative analysis using cryo-TEM and replica freeze-fracture TEM on both thermotropic and lyotropic LCs. In addition to the revisits of previous practices, some new concepts are introduced, e.g., suspended thermotropic LC thin films, combined high-pressure freezing and cryo-sectioning of lyotropic LCs, and the complementary applications of direct TEM and indirect replica TEM techniques. The significance of subnanometer resolution cryo-TEM observation is demonstrated in a few important issues in LC studies, including providing direct evidences for the existence of nanoscale smectic domains in nematic bent-core thermotropic LCs, comprehensive understanding of the twist-bend nematic phase, and probing the packing of columnar aggregates in lyotropic chromonic LCs. Direct TEM observation opens ways to a variety of TEM techniques, suggesting that TEM (replica, cryo, and in situ techniques), in general, may be a promising part of the solution to the lack of effective structural probe at the molecular scale in LC studies. Microsc. Res. Tech. 77:754-772, 2014. (c) 2014 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | Wiley | - |
dc.relation.isPartOf | Microscopy research and technique | - |
dc.title | Direct observation of liquid crystals using cryo‐TEM: Specimen preparation and low‐dose imaging | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jemt.22397 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Microscopy research and technique, v.77, no.10, pp.754 - 772 | - |
dc.identifier.wosid | 000342207900002 | - |
dc.citation.endPage | 772 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 754 | - |
dc.citation.title | Microscopy research and technique | - |
dc.citation.volume | 77 | - |
dc.contributor.affiliatedAuthor | KIM, YOUNGKI | - |
dc.identifier.scopusid | 2-s2.0-84908897413 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TRANSMISSION ELECTRON-MICROSCOPY | - |
dc.subject.keywordPlus | FREEZE-FRACTURE | - |
dc.subject.keywordPlus | PHASE-BEHAVIOR | - |
dc.subject.keywordPlus | DIFFRACTION | - |
dc.subject.keywordPlus | CRYOFIXATION | - |
dc.subject.keywordPlus | TRANSITION | - |
dc.subject.keywordPlus | MESOPHASES | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | DEFECTS | - |
dc.subject.keywordAuthor | freeze fracture | - |
dc.subject.keywordAuthor | high-pressure freezing | - |
dc.subject.keywordAuthor | CEMOVIS | - |
dc.subject.keywordAuthor | thermotropic | - |
dc.subject.keywordAuthor | lyotropic | - |
dc.relation.journalWebOfScienceCategory | Anatomy & Morphology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Microscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Anatomy & Morphology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Microscopy | - |
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