Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids
SCIE
SCOPUS
- Title
- Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids
- Authors
- Garcia-Vello, Pilar; Sharma, Garima; Speciale, Immacolata; Molinaro, Antonio; Im, Sin-Hyeog; De Castro, Cristina
- Date Issued
- 2020-04
- Publisher
- ELSEVIER SCI LTD
- Abstract
- The capsular material from Lactobacillus plantarum IMB19, an isolate from fermented vegetables, has been analyzed and our results demonstrate that most of the coat of this bacterium consists of glycerol- and ribitol-type teichoic acids, further decorated with other substituents (a-glucose and alanine), and of a capsular polysaccharide (CPS) with a linear nonasaccharide repeating unit, rich in rhamnose, interconnected to the next via a phosphodiester bridge. Stimulation of immune cells with the total capsular material resulted in the enhancement of immunostimulatory (IFN gamma, TNF-alpha, IL-6 and IL-12) or immuno-regulatory (IL-10) cytokines in an in vitro splenocyte culture system. The capsular polysaccharide, and not the teichoic acids mixture, was responsible for the IFN gamma production. Indeed, a significant increase of IFN gamma along with other inflammatory cytokines, and a dose response in IFN. expression with an EC50 of 3.16 mu M was found for CPS, disclosing that this polysaccharide is a potent immunostimulatory molecule.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/103623
- DOI
- 10.1016/j.carbpol.2020.115857
- ISSN
- 0144-8617
- Article Type
- Article
- Citation
- CARBOHYDRATE POLYMERS, vol. 233, 2020-04
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- There are no files associated with this item.
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