Design of mutualistic microbial consortia for stable conversion of carbon monoxide to value-added chemicals
SCIE
SCOPUS
- Title
- Design of mutualistic microbial consortia for stable conversion of carbon monoxide to value-added chemicals
- Authors
- Sanghak Cha; Hyun Gyu Lim; Seokmu Kwon; Dong-hwan Kim; Chae Won Kang; Gyoo Yeol Jung
- Date Issued
- 2021-03
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- Carbon monoxide (CO) is a promising carbon source for producing value-added biochemicals via microbial fermentation. However, its microbial conversion has been challenging because of difficulties in genetic engineering of CO-utilizing microorganisms and, more importantly, maintaining CO consumption which is negatively affected by the toxicity of CO and accumulated byproducts. To overcome these issues, we devised mutualistic microbial consortia, co-culturing Eubacterium limosum and genetically engineered Escherichia coli for the production of 3-hydroxypropionic acid (3-HP) and itaconic acid (ITA). During the co-culture, E. limosum assimilated CO and produced acetate, a toxic by-product, while E. coli utilized acetate as a sole carbon source. We found that this mutualistic interaction dramatically stabilized and improved CO consumption of E. limosum compared to monoculture. Consequently, the improved CO consumption allowed successful production of 3-HP and ITA from CO. This study is the first demonstration of value-added biochemical production from CO using a microbial consortium. Moreover, it suggests that synthetic mutualistic microbial consortium can serve as a powerful platform for the valorization of CO.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/105147
- DOI
- 10.1016/j.ymben.2021.02.001
- ISSN
- 1096-7176
- Article Type
- Article
- Citation
- METABOLIC ENGINEERING, vol. 64, page. 146 - 153, 2021-03
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