3D Bioprinting of Multi-Scale Encapsulation System for Islet Transplantation
- Title
- 3D Bioprinting of Multi-Scale Encapsulation System for Islet Transplantation
- Authors
- DONG, GYU HWANG; YEONG, GWON JO; UIJUNG, YONG; CHO, SEUNGYEON; MYUNGJI, KIM; KIMJAEWOOK; CHOI, YOO MI; JANG, JIN AH
- Date Issued
- 2020-08-28
- Publisher
- 한국조직공학 재생의학회 학술대회
- Abstract
- Islet transplantation is a promising treatment for Type 1
diabetes (T1D). However, there are clinical limitations such as
cell dispersion, hypoxia, and infl ammatory response, which lead
to loss of cell functions. Islet encapsulation has been studied
to overcome these limitations. Macroencapsulation refers to
encapsulating a large volume of islets in one system so that it is
retrievable. However, this system has a low surface-to-volume
ratio, which interferes with the diffusion of oxygen and nutrients.
Here, we suggest a 3D bioprinting strategy for a one-step
fabrication of a multi-scale encapsulation system. The developed
3D bioprinted system has both features of macroencapsulation
and microencapsulation systems. The polycaprolactone (PCL)
construct acts as a macroencapsulation system by protecting
internal engineered pancreatic tissue and allow it retrievable. In
addition, engineered pancreatic tissue that is printed directly
into the PCL encapsulation system using a pancreatic tissuederived
decellularized extracellular matrix (pdECM) bioink serves
as a microencapsulation system. The engineered pancreatic
tissue contains cells fabricated into the aggregates to mimic
the native islet morphology, and these aggregates are placed at the demanding position to prevent clumping of cell aggregates
and to a have high surface-to-volume ratio. It was confirmed
that the pore characteristics of the PCL staggered membrane
retained the viability and function of the encapsulated cells
while presenting a reduced pore size. Moreover, the mitigation
of infl ammatory response was investigated in vitro by measuring
infl ammatory cytokine secretion of macrophages, and in vivo by
subcutaneous implantation into Sprague Dawley rats. Together
with this, beta cell aggregates were designed to contain 500
pancreatic β cells with 250 μm diameter. These aggregates
showed reduced hypoxia-induced apoptosis compared to the
non-printed group having the same cell concentration with a
large volume. Also, the aggregates showed earlier expression
of E-cadherin, cell-cell adhesion molecule related to the
maintenance of β -cell viability, and promoting insulin secretion,
than non-printed groups. The results of this study suggest the
possibility of 3D printing for manufacturing islet encapsulation
system, and it could also be applied for cell or tissue delivery
(e.g., adrenal cell, Leydig cell, parathyroid, and thyroid gland)
for treatment of other endocrine diseases. Further research
would be undertaken to investigate the applicability of induced
pluripotent stem cell-derived insulin-producing cells to this
system and its ability to regulate blood sugar levels.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/105999
- Article Type
- Conference
- Citation
- 제21차 한국조직공학 재생의학회 학술대회, page. 145 - 145, 2020-08-28
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