DC Field | Value | Language |
---|---|---|
dc.contributor.author | H. Kim | - |
dc.contributor.author | H. Han | - |
dc.contributor.author | J. Ahn | - |
dc.contributor.author | LEE, JOONGOO | - |
dc.contributor.author | N. Cho | - |
dc.contributor.author | H. Jang | - |
dc.contributor.author | H. Kim | - |
dc.contributor.author | S. Kwon | - |
dc.contributor.author | D. Bang | - |
dc.date.accessioned | 2021-06-11T04:50:29Z | - |
dc.date.available | 2021-06-11T04:50:29Z | - |
dc.date.created | 2021-03-15 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/106613 | - |
dc.description.abstract | We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster. | - |
dc.language | English | - |
dc.publisher | Oxford University Press | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.title | ‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/nar/gks546 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.40, no.18 | - |
dc.identifier.wosid | 000309927100004 | - |
dc.citation.number | 18 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 40 | - |
dc.contributor.affiliatedAuthor | LEE, JOONGOO | - |
dc.identifier.scopusid | 2-s2.0-84867546617 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | GENE SYNTHESIS | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDES | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | MICROCHIPS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | GENOME | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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