‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules
SCIE
SCOPUS
- Title
- ‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules
- Authors
- H. Kim; H. Han; J. Ahn; LEE, JOONGOO; N. Cho; H. Jang; H. Kim; S. Kwon; D. Bang
- Date Issued
- 2012-10
- Publisher
- Oxford University Press
- Abstract
- We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/106613
- DOI
- 10.1093/nar/gks546
- ISSN
- 0305-1048
- Article Type
- Article
- Citation
- NUCLEIC ACIDS RESEARCH, vol. 40, no. 18, 2012-10
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- There are no files associated with this item.
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