Non-Invasive Photothermal Strain Imaging of Non-Alcoholic Fatty Liver Disease in Live Animals
SCIE
SCOPUS
- Title
- Non-Invasive Photothermal Strain Imaging of Non-Alcoholic Fatty Liver Disease in Live Animals
- Authors
- Choi, Changhoon; Choi, Wonseok; Kim, Jeesu; Kim, Chulhong
- Date Issued
- 2021-09
- Publisher
- Institute of Electrical and Electronics Engineers
- Abstract
- The prevalence of non-alcoholic fatty liver diseases (NAFLD) has increased steadily over the past decade. Thus, diagnosing NAFLD at the earliest stage, which is a reversible condition, has become increasingly important. Here, photothermal strain imaging (pTSI) is presented as a novel non-invasive tool for NAFLD diagnosis. The pTSI uses ultrasound to detect the difference in thermal strain between fat and water during a light-induced temperature rise, which is directly related to the pathological evidence of NAFLD. To demonstrate its feasibility, fat accumulation in in vivo rat livers is monitored non-invasively using pTSI, based on clinical ultrasound B-mode images. A total of 21 male Wistar rats of 3 weeks of age were prepared. Of these, 18 rats received methionine-choline deficient diet for 1 to 6 weeks (n = 3 per week) to induce NAFLD, whereas 3 rats received normal diet as controls (n = 3). Livers were heated by a lipid-sensitive continuous-wave laser, and strain was measured. Quantitative results from the pTSI were compared with histological analysis results using Oil-Red-O (ORO). The receiver operating characteristic curve of in vivo pTSI results for detecting moderate steatosis (ORO-stained area >= 33%) was constructed based on strain change rate measured in the liver region. The sensitivity and specificity of pTSI were 90% and 82%, respectively, and the area-under-the-curve was measured as 0.85 +/- 0.03 (95% confidence interval). The pTSI results tested in the rodent NAFLD model showed great potential for pTSI to be used as a new diagnostic tool for NAFLD in the future.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/109576
- DOI
- 10.1109/TMI.2021.3081097
- ISSN
- 0278-0062
- Article Type
- Article
- Citation
- IEEE Transactions on Medical Imaging, vol. 40, no. 9, page. 2487 - 2495, 2021-09
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