A Gene-Networked Gel Matrix-Supported Lipid Bilayer as a Synthetic Nucleus System
SCIE
SCOPUS
- Title
- A Gene-Networked Gel Matrix-Supported Lipid Bilayer as a Synthetic Nucleus System
- Authors
- Bae, Sun Ju; Song, Woo Chul; Jung, Sung Hwan; Cho, Seung-Woo; Kim, Dong-Ik; Um, Soong Ho
- Date Issued
- 2012-12
- Publisher
- American Chemical Society
- Abstract
- A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/116036
- DOI
- 10.1021/la303498k
- ISSN
- 0743-7463
- Article Type
- Article
- Citation
- Langmuir, vol. 28, no. 49, page. 17036 - 17042, 2012-12
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.