5'-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
SCIE
SCOPUS
- Title
- 5'-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
- Authors
- Sun-Young Hwang; Hwa-Young Sun; Kwang-Hoon Lee; Byung-Ha Oh; Yu Jin Cha; Byeang Hyean Kim; Yoo, JY
- Date Issued
- 2012-03
- Publisher
- London, Information Retrieval ltd.
- Abstract
- RIG-I is a cytosolic receptor for non-self RNA that mediates immune responses against viral infections through IFN alpha/beta production. In an attempt to identify novel tools that modulate IFN alpha/beta production, we used SELEX technology to screen RNA aptamers that specifically target RIG-I protein. Most of the selected RIG-I aptamers contained polyU motifs in the second half regions that played critical roles in the activation of RIG-I-mediated IFN beta production. Unlike other known ligands, RIG-I aptamer bound and activated RIG-I in a 5'-triphosphate-independent manner. The helicase and RD domain of RIG-I were used for aptamer binding, but intact RIG-I protein was required to exert aptamer-mediated signaling activation. Furthermore, replication of NDV, VSV and influenza virus in infected host cells was efficiently blocked by pre- or post-treatment with RIG-I aptamer. Based on these data, we propose that RIG-I aptamer has strong potential to be an antiviral agent that specifically boosts the RIG-I-dependent signaling cascade.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/11848
- DOI
- 10.1093/NAR/GKR1098
- ISSN
- 0305-1048
- Article Type
- Article
- Citation
- NUCLEIC ACIDS RESEARCH, vol. 40, no. 6, page. 2724 - 2733, 2012-03
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