Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN
SCIE
SCOPUS
- Title
- Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN
- Authors
- Cho Eunbyul; Woo Youngsik; Suh Yeongjun; Suh Bo Kyoung; Kim Soo Jeong; Nhung Truong Thi My; Yoo Jin Yeong; Nghi Tran Diem; Lee Su Been; Mun Dong Jin; Park Sang Ki
- Date Issued
- 2023-06
- Publisher
- Nature Publishing Group
- Abstract
- Mitochondria-associated ER membrane (MAM) is a structure where these calcium-regulating organelles form close physical contact sites for efficient Ca2+ crosstalk. Despite the central importance of MAM Ca2+ dynamics in diverse biological processes, directly and specifically measuring Ca2+ concentrations inside MAM is technically challenging. Here, we develop MAM-Calflux, a MAM-specific BRET-based Ca2+ indicator. The successful application of the bimolecular fluorescence complementation (BiFC) concept highlights Ca2+-responsive BRET signals in MAM. The BiFC strategy imparts dual functionality as a Ca2+ indicator and quantitative structural marker specific for MAM. As a ratiometric Ca2+ indicator, MAM-Calflux estimates steady-state MAM Ca2+ levels. Finally, it enables the visualization of uneven intracellular distribution of MAM Ca2+ and the elucidation of abnormally accumulated MAM Ca2+ from the neurons of Parkinson's disease mouse model in both steady-state and stimulated conditions. Therefore, we propose that MAM-Calflux can be a versatile tool for ratiometrically measuring dynamic inter-organellar Ca2+ communication.,MAM Ca2+ dynamics play an important role in diverse biological processes, but directly and specifically measuring Ca2+ concentrations in this region is technically challenging. Here the authors report a MAM-specific BRET-based Ca2+ indicator called MAM-Calflux, which works as both a Ca2+ indicator and a structural marker due to its ratiometric nature.,
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/118757
- DOI
- 10.1038/s41467-023-39343-2
- Article Type
- Article
- Citation
- Nature Communications, vol. 14, no. 1, 2023-06
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