The immediate upstream region of the 5′-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana
SCIE
SCOPUS
- Title
- The immediate upstream region of the 5′-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana
- Authors
- Younghyun Kim; Goeun Lee; Eunhyun Jeon; Eun Ju Sohn; Yongjik Lee; Hyangju Kang; Dong Wook Lee; Dae Heon Kim; Hwang, I
- Date Issued
- 2014-01
- Publisher
- Oxford University Press
- Abstract
- The nucleotide sequence around the translational initiation site is an important cis-acting element for post-transcriptional regulation. However, it has not been fully understood how the sequence context at the 5'-untranslated region (5'-UTR) affects the translational efficiency of individual mRNAs. In this study, we provide evidence that the 5'-UTRs of Arabidopsis genes showing a great difference in the nucleotide sequence vary greatly in translational efficiency with more than a 200-fold difference. Of the four types of nucleotides, the A residue was the most favourable nucleotide from positions -1 to -21 of the 5'-UTRs in Arabidopsis genes. In particular, the A residue in the 5'-UTR from positions -1 to -5 was required for a high-level translational efficiency. In contrast, the T residue in the 5'-UTR from positions -1 to -5 was the least favourable nucleotide in translational efficiency. Furthermore, the effect of the sequence context in the -1 to -21 region of the 5'-UTR was conserved in different plant species. Based on these observations, we propose that the sequence context immediately upstream of the AUG initiation codon plays a crucial role in determining the translational efficiency of plant genes.
- Keywords
- RNA SECONDARY STRUCTURE; 5' UNTRANSLATED REGION; VERTEBRATE MESSENGER-RNAS; INITIATION CODON; EXPRESSION; PROTEIN; PLANTS; GENE; SEQUENCES; ELEMENT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13093
- DOI
- 10.1093/NAR/GKT864
- ISSN
- 0305-1048
- Article Type
- Article
- Citation
- NUCLEIC ACIDS RESEARCH, vol. 42, no. 1, page. 485 - 498, 2014-01
- Files in This Item:
-
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.