Precise characterization method of antibody-conjugated magnetic nanoparticles for pathogen detection using stuffer-free multiplex ligation-dependent probe amplification
SCIE
SCOPUS
- Title
- Precise characterization method of antibody-conjugated magnetic nanoparticles for pathogen detection using stuffer-free multiplex ligation-dependent probe amplification
- Authors
- Chung, B; Shin, GW; Choi, W; Joo, J; Jeon, S; Jung, GY
- Date Issued
- 2014-12
- Publisher
- WILEY-BLACKWELL
- Abstract
- Antibody-conjugated magnetic nanoparticles (Ab-MNPs) have potential in pathogen detection because they allow target cells to be easily separated from complex sample matrices. However, the sensitivity and specificity of pathogen capture by Ab-MNPs generally vary according to the types of MNPs, antibodies, and sample matrices, as well as preparation methods, including immobilization. Therefore, achieving a reproducible analysis utilizing Ab-MNPs as a pathogen detection method requires accurate characterization of Ab-MNP capture ability and standardization of all handling processes. In this study, we used high-resolution CE-single strand conformational polymorphism coupled with a stuffer-free multiplex ligation-dependent probe amplification system to characterize Ab-MNPs. The capture ability of Ab-MNPs targeting Salmonella enteritidis and nine pathogens, including S. enteritidis, was analyzed in phosphate buffer and milk. The effect of storage conditions on the stability of Ab-MNPs was also assessed. The results showed that the stuffer-free multiplex ligation-dependent probe amplification system has the potential to serve as a standard characterization method for Ab-MNPs. Moreover, the precise characterization of Ab-MNPs facilitated robust pathogen detection in various applications.
- Keywords
- Antibody-conjugated magnetic nanoparticles; Capillary electrophoresis-single-strand conformation polymorphism; Pathogen detection; Stuffer-free multiplex ligation-dependent probe amplification; RESOLUTION CE-SSCP; ESCHERICHIA-COLI O157-H7; FOOD SAMPLES; IMPEDANCE BIOSENSOR; SENSITIVE DETECTION; BACTERIA; SEPARATION; SENSOR; PCR
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13914
- DOI
- 10.1002/ELPS.201400180
- ISSN
- 0173-0835
- Article Type
- Article
- Citation
- ELECTROPHORESIS, vol. 35, no. 23, page. 3283 - 3289, 2014-12
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