Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds
SCIE
SCOPUS
- Title
- Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds
- Authors
- Kim, CS; Seo, JH; Kang, DG; Cha, HJ
- Date Issued
- 2014-05
- Publisher
- ELSEVIER
- Abstract
- The development of efficient tools is required for the eco-friendly detoxification and effective detection of neurotoxic organophosphates (OPs). Although enzymes have received significant attention as biocatalysts because of their high specific activity, the uneconomic and labor-intensive processes of enzyme production and purification make their broad use in practical applications difficult. Because whole7cell systems offer several advantages compared with free enzymes, including high stability, a reduced purification requirement, and low preparation cost, they have been suggested as promising biocatalysts for the detoxification and detection of OPs. To develop efficient whole-cell biocatalysts with enhanced activity and a broad spectrum of substrate specificity, several factors have been considered, namely the selected strains, the chosen OP-hydrolyzing enzymes, where enzymes are localized in a cell, and which enhancer will assist the expression, function, and folding of the enzyme. In this article, we review the current investigative progress in the development of engineered whole-cell biocatalysts with excellent OP-hydrolyzing activity, a broad spectrum of substrate specificity, and outstanding stability for the detoxification and detection of OPs. (C) 2014 Elsevier Inc. All rights reserved.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/14238
- DOI
- 10.1016/J.BIOTECHADV.2014.04.010
- ISSN
- 0734-9750
- Article Type
- Article
- Citation
- BIOTECHNOLOGY ADVANCES, vol. 32, no. 3, page. 652 - 662, 2014-05
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