Parallel recordings of photosynthetic electron transport and K+-channel activity in single guard cells
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SCOPUS
- Title
- Parallel recordings of photosynthetic electron transport and K+-channel activity in single guard cells
- Authors
- Goh, CH; Dietrich, P; Steinmeyer, R; Schreiber, U; Nam, HG; Hedrich, R
- Date Issued
- 2002-11
- Publisher
- BLACKWELL PUBLISHING LTD
- Abstract
- Stomata open in response to red and blue light. Red light-induced stomatal movement is mediated by guard cell chloroplasts and related to K+-uptake into these motor cells. We have combined a new type of microchlorophyll fluorometer with the patch-clamp technique for parallel studies of the photosynthetic electron transport and activity of plasma membrane K+ channels in single guard cell protoplast. In the whole-cell configuration and presence of ATP in the patch-pipette, the activity of the K+-uptake channels remained constant throughout the course of an experiment (up to 30 min) while photosynthetic activity declined to about 50%. In the absence of ATP inward K+ currents declined in a time-dependent manner. Under these ATP-free conditions, photosynthetic electron transport was completely blocked within 8 min. ADP together with orthophosphate was able to prevent inhibition of photosynthetic electron transport and run-down of K+-channel activity. The results demonstrate that the combination of these two techniques is suited to directly study cytosolic factors as common regulators of photosynthesis and plasma membrane transport within a single-cell.
- Keywords
- ATP; chlorophyll fluorescence; electron transport; guard cell; K+ channel; patch-clamp; PLASMA-MEMBRANE; VOLTAGE-DEPENDENCE; KAT1 CHANNEL; PROTON PUMP; PLANT-CELL; PROTOPLASTS; LIGHT; MODULATION; HYPERPOLARIZATION; ACIDIFICATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18810
- DOI
- 10.1046/j.1365-313X.2002.01451.x
- ISSN
- 0960-7412
- Article Type
- Article
- Citation
- PLANT JOURNAL, vol. 32, no. 4, page. 623 - 630, 2002-11
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