DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dharuman, V | - |
dc.contributor.author | Hahn, JH | - |
dc.date.accessioned | 2016-04-01T01:23:44Z | - |
dc.date.available | 2016-04-01T01:23:44Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2008-03-14 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.other | 2008-OAK-0000007646 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/22833 | - |
dc.description.abstract | Electrochemical label free DNA hybridization discrimination of the brain tumor sequence CK20 has been made at the gold-thiol and thiol diluent binary and ternary mixed monolayer interfaces in presence of the [Fe(CN)(6)](3-) and double stranded DNA (dsDNA) specific cationic intercalators, proflavine (PF) and methylene blue (MB), respectively. Thiol hexane labeled single stranded DNA (HS-ssDNA) and thiol diluents such as 6-mercapto-1-hexanol (MCH) and 3-mercaptopropionic acid (MPA) are used to construct the mixed monolayers. Change in the peak-to-peak separation (Delta E-p) for the [Fe(CN)(6)](3-) redox reaction indicates the efficiency of the diluents in removing the randomly oriented HS-ssDNA. Smaller AEp 248 mV noticed for the HS-ssDNA/MPA compared to the HS-ssDNA/MCH mixed monolayers (812 mV) indicates the less influence of the MCH diluent on the arrangement of HS-ssDNA layer. However, the hybridization discrimination effect negotiated in presence of both the [Fe(CN)(6)](3-) and PF intercalator showed zero effect for the HS-ssDNA/MPA interface, and similar to 20-30% effect for the HS-ssDNA/MCH interface. The discrimination effect at the HS-ssDNA/MPA interface further increased to 80% by inserting the MCH at the local defects to form a multicomponent ternary HS-ssDNA/MPA/MCH layer interface. These differential discrimination effects are attributed to the formation of compact and/or defective layer structures, evidenced from their reductive desorption voltammetry in 0.5 M KOH. The presence of single base (C-A) mismatch in the hybrid is diagnosed by a decrease in coulometric charge compared to the perfect dsDNA. The target concentration of 10pM is detected selectively and sensitively. (c) 2007 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.subject | DNA hybridization effect | - |
dc.subject | electrochemical | - |
dc.subject | label free | - |
dc.subject | binary | - |
dc.subject | ternary mixed monolayers | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | MEDIATED CHARGE-TRANSPORT | - |
dc.subject | 3-MERCAPTOPROPIONIC ACID | - |
dc.subject | IMPEDANCE SPECTROSCOPY | - |
dc.subject | CYCLIC VOLTAMMETRY | - |
dc.subject | ELECTRON-TRANSFER | - |
dc.subject | PHASE-SEPARATION | - |
dc.subject | GOLD ELECTRODES | - |
dc.subject | METHYLENE-BLUE | - |
dc.subject | SELECTIVE REPLACEMENT | - |
dc.title | Label free electrochemical DNA hybridization discrimination effects at the binary and ternary mixed monolayers of single stranded DNA/diluent/s in presence of cationic intercalators | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1016/j.bios.2007.11.015 | - |
dc.author.google | Dharuman, V | - |
dc.author.google | Hahn, JH | - |
dc.relation.volume | 23 | - |
dc.relation.issue | 8 | - |
dc.relation.startpage | 1250 | - |
dc.relation.lastpage | 1258 | - |
dc.contributor.id | 10082554 | - |
dc.relation.journal | BIOSENSORS & BIOELECTRONICS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.23, no.8, pp.1250 - 1258 | - |
dc.identifier.wosid | 000254691300005 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1258 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1250 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 23 | - |
dc.contributor.affiliatedAuthor | Hahn, JH | - |
dc.identifier.scopusid | 2-s2.0-39649105438 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 36 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | MEDIATED CHARGE-TRANSPORT | - |
dc.subject.keywordPlus | 3-MERCAPTOPROPIONIC ACID | - |
dc.subject.keywordPlus | GOLD ELECTRODES | - |
dc.subject.keywordPlus | METHYLENE-BLUE | - |
dc.subject.keywordPlus | SELECTIVE REPLACEMENT | - |
dc.subject.keywordPlus | SURFACE-STRUCTURE | - |
dc.subject.keywordPlus | PHASE-SEPARATION | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | ALKANETHIOLS | - |
dc.subject.keywordAuthor | DNA hybridization effect | - |
dc.subject.keywordAuthor | electrochemical | - |
dc.subject.keywordAuthor | label free | - |
dc.subject.keywordAuthor | binary | - |
dc.subject.keywordAuthor | ternary mixed monolayers | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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