Label free electrochemical DNA hybridization discrimination effects at the binary and ternary mixed monolayers of single stranded DNA/diluent/s in presence of cationic intercalators
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SCOPUS
- Title
- Label free electrochemical DNA hybridization discrimination effects at the binary and ternary mixed monolayers of single stranded DNA/diluent/s in presence of cationic intercalators
- Authors
- Dharuman, V; Hahn, JH
- Date Issued
- 2008-03-14
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Abstract
- Electrochemical label free DNA hybridization discrimination of the brain tumor sequence CK20 has been made at the gold-thiol and thiol diluent binary and ternary mixed monolayer interfaces in presence of the [Fe(CN)(6)](3-) and double stranded DNA (dsDNA) specific cationic intercalators, proflavine (PF) and methylene blue (MB), respectively. Thiol hexane labeled single stranded DNA (HS-ssDNA) and thiol diluents such as 6-mercapto-1-hexanol (MCH) and 3-mercaptopropionic acid (MPA) are used to construct the mixed monolayers. Change in the peak-to-peak separation (Delta E-p) for the [Fe(CN)(6)](3-) redox reaction indicates the efficiency of the diluents in removing the randomly oriented HS-ssDNA. Smaller AEp 248 mV noticed for the HS-ssDNA/MPA compared to the HS-ssDNA/MCH mixed monolayers (812 mV) indicates the less influence of the MCH diluent on the arrangement of HS-ssDNA layer. However, the hybridization discrimination effect negotiated in presence of both the [Fe(CN)(6)](3-) and PF intercalator showed zero effect for the HS-ssDNA/MPA interface, and similar to 20-30% effect for the HS-ssDNA/MCH interface. The discrimination effect at the HS-ssDNA/MPA interface further increased to 80% by inserting the MCH at the local defects to form a multicomponent ternary HS-ssDNA/MPA/MCH layer interface. These differential discrimination effects are attributed to the formation of compact and/or defective layer structures, evidenced from their reductive desorption voltammetry in 0.5 M KOH. The presence of single base (C-A) mismatch in the hybrid is diagnosed by a decrease in coulometric charge compared to the perfect dsDNA. The target concentration of 10pM is detected selectively and sensitively. (c) 2007 Elsevier B.V. All rights reserved.
- Keywords
- DNA hybridization effect; electrochemical; label free; binary; ternary mixed monolayers; SELF-ASSEMBLED MONOLAYERS; MEDIATED CHARGE-TRANSPORT; 3-MERCAPTOPROPIONIC ACID; IMPEDANCE SPECTROSCOPY; CYCLIC VOLTAMMETRY; ELECTRON-TRANSFER; PHASE-SEPARATION; GOLD ELECTRODES; METHYLENE-BLUE; SELECTIVE REPLACEMENT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22833
- DOI
- 10.1016/j.bios.2007.11.015
- ISSN
- 0956-5663
- Article Type
- Article
- Citation
- BIOSENSORS & BIOELECTRONICS, vol. 23, no. 8, page. 1250 - 1258, 2008-03-14
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