Crif1 is a novel transcriptional coactivator of STAT3
SCIE
SCOPUS
- Title
- Crif1 is a novel transcriptional coactivator of STAT3
- Authors
- Kwon, MC; Koo, BK; Moon, JS; Kim, YY; Park, KC; Kim, NS; Kwon, MY; Kong, MP; Yoon, KJ; Im, SK; Ghim, J; Han, YM; Jang, SK; Shong, M; Kong, YY
- Date Issued
- 2008-02-20
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- Signal transducer and activator of transcription 3 (STAT3) is a transcriptional factor that performs a broad spectrum of biological functions in response to various stimuli. However, no specific coactivator that regulates the transcriptional activity of STAT3 has been identified. Here we report that CR6-interacting factor 1 (Crif1) is a specific transcriptional coactivator of STAT3, but not of STAT1 or STAT5a. Crif1 interacts with STAT3 and positively regulates its transcriptional activity. Crif1(-/-) embryos were lethal around embryonic day 6.5, and manifested developmental arrest accompanied with defective proliferation and massive apoptosis. The expression of STAT3 target genes was markedly reduced in a Crif1(-/-) blastocyst culture and in Oncostatin M-stimulated Crif1-deficient MEFs. Importantly, the key activities of constitutively active STAT3-C, such as transcription, DNA binding, and cellular transformation, were abolished in the Crif1-null MEFs, suggesting the essential role of Crif1 in the transcriptional activity of STAT3. Our results reveal that Crif1 is a novel and essential transcriptional coactivator of STAT3 that modulates its DNA binding ability, and shed light on the regulation of oncogenic STAT3.
- Keywords
- Crif1; embryogenesis; STAT3; transcriptional coactivator; tumorigenesis; CR6-INTERACTING FACTOR-1 INTERACTS; COILED-COIL DOMAIN; FUNCTIONAL INTERACTION; TARGETED DISRUPTION; SIGNAL-TRANSDUCTION; MICE LACKING; ACTIVATION; PROTEINS; CELLS; GENE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22906
- DOI
- 10.1038/sj.emboj.7601986
- ISSN
- 0261-4189
- Article Type
- Article
- Citation
- EMBO JOURNAL, vol. 27, no. 4, page. 642 - 653, 2008-02-20
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.