Simple Screening Method for Double-strand DNA Binders Using Hairpin DNA-modified Magnetic Beads
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- Title
- Simple Screening Method for Double-strand DNA Binders Using Hairpin DNA-modified Magnetic Beads
- Authors
- Jo, H; Min, K; Song, KM; Ku, JK; Han, MS; Ban, C
- Date Issued
- 2011-01-20
- Publisher
- KOREAN CHEMICAL SOC
- Abstract
- We designed an effective screening method for double strand DNA (dsDNA) binders using DNA-modified magnetic particles. Hairpin DNA was immobilized on the surface of magnetic particle for a simple screening of dsDNA binding materials in a solution containing various compounds. Through several magnetic separation and incubation processes, four DNA-binding materials, DAPI, 9AA, AQ2A, and DNR, were successfully screened from among five candidates. Efficiency of screening was demonstrated by HPLC analysis using a C2/18 reverse-phase column. In addition, their relative binding strengths were verified by measuring the melting temperature (T-m). If hairpin DNA sequence is modified for other uses, this magnetic bead-based approach can be applied as a high-throughput screening method for various functional materials such as anti-cancer drugs.
- Keywords
- Screening; dsDNA binder; Magnetic bead; Hairpin DNA; HPLC; ANTICANCER DRUG SCREEN; SMALL-MOLECULE; COMBINATORIAL APPROACH; NANOPARTICLE PROBES; CANCER-CELLS; TUMOR-CELLS; BINDING; AGENTS; DOXORUBICIN; COMPLEXES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25093
- DOI
- 10.5012/BKCS.2011.32.1.247
- ISSN
- 0253-2964
- Article Type
- Article
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, vol. 32, no. 1, page. 247 - 250, 2011-01-20
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- There are no files associated with this item.
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