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A Surface Forces Study of DNA Hybridization SCIE SCOPUS

Title
A Surface Forces Study of DNA Hybridization
Authors
Cho, YKKim, SLim, GGranick, S
Date Issued
2001-12-11
Publisher
AMER CHEMICAL SOC
Abstract
We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (approximate to1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.
Keywords
MONOLAYERS; GOLD
URI
https://oasis.postech.ac.kr/handle/2014.oak/25487
DOI
10.1021/la0113769
ISSN
0743-7463
Article Type
Article
Citation
LANGMUIR, vol. 17, no. 25, page. 7732 - 7734, 2001-12-11
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임근배LIM, GEUN BAE
Dept of Mechanical Enginrg
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