Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA
SCIE
SCOPUS
- Title
- Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA
- Authors
- Namgung, R; Brumbach, JH; Jeong, JH; Yockman, JW; Kim, SW; Lin, C; Zhong, ZY; Feijen, J; Engbersen, JFJ; Kim, WJ
- Date Issued
- 2010-06
- Publisher
- SPRINGER
- Abstract
- A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems.
- Keywords
- Dual bio-responsive; Gene therapy; Reducible polymer; Survivin; Tumor cells; ONCOLYTIC ADENOVIRUS; DISULFIDE LINKAGES; MOUSE SURVIVIN; CANCER; APOPTOSIS; PROMOTER; INHIBITION; EXPRESSION; COMPLEXES; THERAPY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25597
- DOI
- 10.1007/S10529-010-0219-7
- ISSN
- 0141-5492
- Article Type
- Article
- Citation
- BIOTECHNOLOGY LETTERS, vol. 32, no. 6, page. 755 - 764, 2010-06
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