Triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection
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SCOPUS
- Title
- Triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection
- Authors
- Kim, SJ; Shin, GW; Choi, SJ; Hwang, HS; Jung, GY; Seo, TS
- Date Issued
- 2010-03
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- Rapid and simple analysis for the multiple target pathogens is critical for patient management CE-SSCP analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields The capability of separating ssDNA molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram In this study, we explored the potential of poly(ethyleneoxide)-poly (propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer as a sieving matrix for CE-SSCP analysis on a microdevice To demonstrate the superior resolving power of PEO-PPO-PEO copolymers, 255-bp PCR amplicons obtained from 16S ribosomal RNA genes of four bacterial species, namely Proteus mirabilis, Haemophilus ducreyt, Pseudomonas aerugmosa, and Neisseria meningitidis, were analyzed in the PEO-PPO-PEO matrix in comparison with 5% linear polyacrylamide and commercial GeneScan (TM) gel. Due to enhanced dynamic coating and sieving ability, PEO-PPO-PEO copolymer displayed fourfold enhancement of resolving power in the CE-SSCP to separate same-sized DNA molecules Fivefold Input of genomic DNA of P aeruginosa and/or N meningitidis produced proportionally increased corresponding amplicon peaks, enabling correct quantitative analysis in the pathogen detection. Besides the high-resolution sieving capability, a facile loading and replenishment of gel in the microchannel due to thermally reversible gelation property makes PEO-PPO-PEO triblock copolymer an excellent matrix in the CE-SSCP analysis on the microdevice
- Keywords
- CE-SSCP; Microdevice; Pathogen; Poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide); Quantification; STRAND CONFORMATION POLYMORPHISM; MICROCHIP ELECTROPHORESIS; FOODBORNE PATHOGENS; BLOCK-COPOLYMERS; PCR; SEPARATION; SYSTEM; GENE; SSCP; CE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25876
- DOI
- 10.1002/ELPS.200900651
- ISSN
- 0173-0835
- Article Type
- Article
- Citation
- ELECTROPHORESIS, vol. 31, no. 6, page. 1108 - 1115, 2010-03
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