A lysosomal tetraspanin associated with retinal degeneration identified via a genome-wide screen
SCIE
SCOPUS
- Title
- A lysosomal tetraspanin associated with retinal degeneration identified via a genome-wide screen
- Authors
- Xu, H; Lee, SJ; Suzuki, E; Dugan, KD; Stoddard, A; Li, HS; Chodosh, LA; Montell, C
- Date Issued
- 2004-02-25
- Publisher
- European Molecular Biology Organization Press
- Abstract
- The Drosophila visual system has provided a model to study phototransduction and retinal degeneration. To identify new candidate proteins that contribute to these processes, we conducted a genome-wide screen for genes expressed predominately in the eye, using DNA microarrays. This screen appeared to be comprehensive as it led to the identification of all 22 eye-enriched genes previously shown to function in phototransduction or implicated in retinal degeneration. In addition, we identified 93 eye-enriched genes whose roles have not been previously defined. One of the eye-enriched genes encoded a member of a large family of transmembrane proteins, referred to as tetraspanins. We created a null mutation in the eye-enriched tetraspanin, Sunglasses (Sun), which resulted in light-induced retinal degeneration. We found that the Sun protein was distributed primarily in lysosomes, and functioned in a long-known but poorly understood phenomenon of light-induced degradation of rhodopsin. We propose that lysosomal tetraspanins in mammalian cells may also function in the downregulation of rhodopsin and other G-protein-coupled receptors, in response to intense or prolonged agonist stimulation.
- Keywords
- Drosophila; microarray; phototransduction; retinal degeneration; rhodopsin; tetraspanin; DROSOPHILA-MELANOGASTER; GENE-EXPRESSION; PHOTORECEPTOR CELLS; PROTEIN; ARRESTIN; COMPLEX; CDNA; INAD; TRP; TRANSLOCATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/27586
- DOI
- 10.1038/sj.emboj.7600112
- ISSN
- 0261-4189
- Article Type
- Article
- Citation
- The EMBO Journal, vol. 23, no. 4, page. 811 - 822, 2004-02-25
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.