STABILIZATION EFFECT OF ZEOLITE ON DHFR MRNA IN A WHEAT GERM CELL-FREE PROTEIN SYNTHESIS SYSTEM
SCIE
SCOPUS
- Title
- STABILIZATION EFFECT OF ZEOLITE ON DHFR MRNA IN A WHEAT GERM CELL-FREE PROTEIN SYNTHESIS SYSTEM
- Authors
- Jung, GY; Lee, EY; Kim, YE; Jung, BW; Kang, SH; Choi, CY
- Date Issued
- 2000-02
- Publisher
- SOC BIOSCIENCE BIOENGINEERING JAPAN
- Abstract
- The effects of zeolites and monocations on the protein synthesis in a cell-free system derived from wheat germ were investigated. M type of synthetic zeolite markedly enhanced the translation efficiency. Whereas this kind of stimulatory effect of zeolite in an Escherichia coli cell-free system resulted from a change in the salt compositions of the reaction solution with the addition of zeolite, the enhancement of protein synthesis in a wheat germ cell-free system was not due to the ion exchange reaction of zeolites. From the results of mRNA stability analysis, it was found that zeolite could stabilize the mRNA in a wheat germ cell-free protein synthesis system. The stabilization of mRNA by the simple addition of zeolites is useful for the enhancement of protein synthesis in a wheat germ cell-free system, since conventional methods to improve mRNA stability, such as the addition of nuclease inhibitor, have not been effective for a wheat germ cell-free system.
- Keywords
- cell-free protein synthesis system; wheat germ; mRNA; stabilization
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28361
- DOI
- 10.1016/S1389-1723(00)88736-8
- ISSN
- 1389-1723
- Article Type
- Article
- Citation
- JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 89, no. 2, page. 193 - 195, 2000-02
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