Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single-Strand Conformation Polymorphism
SCOPUS
- Title
- Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single-Strand Conformation Polymorphism
- Authors
- Hwang H.S; Shin G.W; Chung B; Na J; Jung G.Y.
- Date Issued
- 2013-01
- Publisher
- Humana press
- Abstract
- Among the molecular diagnostic methods for bacteria-induced diseases, capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR has enormous potential because it can separate sequence variants using a simple procedure. However, conventional CE-SSCP systems have limited resolution and cannot separate most 16S rRNA gene-specific markers into separate peaks. A high-resolution CE-SSCP system that uses a poly(ethyleneoxide)-poly(propyleneoxide)- poly(ethyleneoxide) triblock copolymer matrix was recently developed and shown to effectively separate highly similar PCR products. In this report, a protocol for the detection of 12 pathogenic bacteria is provided. Pathogen markers were amplified by PCR using universal primers and separated by CE-SSCP; each marker peak was well separated at baseline and showed a characteristic mobility, allowing the easy identification of the pathogens.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/34665
- DOI
- 10.1007/978-1-62703-029-8_15
- ISSN
- 1940-6029
- Article Type
- Article
- Citation
- Methods in Molecular Biology, vol. 919, page. 155 - 163, 2013-01
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- There are no files associated with this item.
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