Four cDNAs encoding lipoprotein receptors from shrimp (Pandalopsis japonicus): Structural characterization and expression analysis during maturation
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- Title
- Four cDNAs encoding lipoprotein receptors from shrimp (Pandalopsis japonicus): Structural characterization and expression analysis during maturation
- Authors
- Lee, JH; Kim, BK; Seo, YI; Choi, JH; Kang, SW; Kang, CK; Park, WG; Kim, HW
- Date Issued
- 2014-03
- Publisher
- Elsevier
- Abstract
- As in all other oviparous animals, lipoprotein receptors play a critical role in lipid metabolism and reproduction in decapod crustaceans. Four full-length cDNAs encoding lipoprotein receptors (Paj-VgR, Paj-LpR1, Paj-LpR2A, and Paj-LpR2B) were identified from Pandalopsis japonica through a combination of EST screening and PCR-based cloning. Paj-LpR1 appears to be the first crustacean ortholog of insect lipophorin receptors, and its two paralogs, Paj-LpR2A and Paj-LpR2B, exhibited similar structural characteristics. Several transcriptional isoforms were also identified for all three Paj-LpRs. Each expression pattern was unique, suggesting different physiological roles for these proteins. Paj-VgR is an ortholog of vitellogenin (Vg) receptors from other decapod crustaceans. A phylogenetic analysis of lipoproteins and their receptors suggested that the nomenclature of Vgs from decapod crustaceans may need to be changed. A PCR-based transcriptional analysis showed that Paj-VgR and Paj-LpR2B are expressed almost exclusively in the ovary, whereas Paj-LpR1 and Paj-LpR2A are expressed in multiple tissues. The various transcriptional isoforms of the three Paj-LpRs exhibited unique tissue distribution profiles. A transcriptional analysis of each receptor using tissues with different GSI values showed that the change in transcription of Paj-VgRs, Paj-LpR2A and Paj-LpR1 was not as significant as that of Vgs during maturation. However, the transcriptional levels of Paj-LpR2B decreased in ovary at maturation, suggesting that their transcriptional regulation is involved in reproduction. (C) 2013 Elsevier Inc All rights reserved.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/35304
- DOI
- 10.1016/J.CBPB.2013.12.005
- ISSN
- 1096-4959
- Article Type
- Article
- Citation
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, vol. 169, page. 51 - 62, 2014-03
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